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Title: | Hyaluronic acid-based bioink improves the differentiation and network formation of neural progenitor cells |
Author: | Pereira, Inês López Martínez, María José Villasante, Aranzazu Introna, Clelia Tornero, Daniel Canals i Coll, Josep M. Samitier i Martí, Josep |
Keywords: | Materials biomèdics Impressió 3D Àcid hialurònic Diferenciació de productes Regeneració del sistema nerviós Biomedical materials Three-dimensional printing Hyaluronic acid Product differentiation Nervous system regeneration |
Issue Date: | 3-Mar-2023 |
Publisher: | Frontiers Media |
Abstract: | Introduction: Three-dimensional (3D) bioprinting is a promising technique for the development of neuronal in vitro models because it controls the deposition of materials and cells. Finding a biomaterial that supports neural differentiation in vitro while ensuring compatibility with the technique of 3D bioprinting of a self-standing construct is a challenge. Methods: In this study, gelatin methacryloyl (GelMA), methacrylated alginate (AlgMA), and hyaluronic acid (HA) were examined by exploiting their biocompatibility and tunable mechanical properties to resemble the extracellular matrix (ECM) and to create a suitable material for printing neural progenitor cells (NPCs), supporting their long-term differentiation. NPCs were printed and differentiated for up to 15 days, and cell viability and neuronal differentiation markers were assessed throughout the culture. Results and Discussion: This composite biomaterial presented the desired physical properties to mimic the ECM of the brain with high water intake, low stiffness, and slow degradation while allowing the printing of defined structures. The viability rates were maintained at approximately 80% at all time points. However, the levels of β-III tubulin marker increased over time, demonstrating the compatibility of this biomaterial with neuronal cell culture and differentiation. Furthermore, these cells showed increased maturation with corresponding functional properties, which was also demonstrated by the formation of a neuronal network that was observed by recording spontaneous activity via Ca2+ imaging. |
Note: | Reproducció del document publicat a: https://doi.org/10.3389/fbioe.2023.1110547 |
It is part of: | Frontiers In Bioengineering And Biotechnology, 2023, vol. 11, p. 1110547 |
URI: | http://hdl.handle.net/2445/195645 |
Related resource: | https://doi.org/10.3389/fbioe.2023.1110547 |
ISSN: | 2296-4185 |
Appears in Collections: | Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) Articles publicats en revistes (Institut de Neurociències (UBNeuro)) Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) Articles publicats en revistes (Biomedicina) |
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