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http://hdl.handle.net/2445/196118
Title: | Treatment with integrase inhibitors alters SARS-CoV-2 neutralization levels measured with HIV-based pseudotypes in people living with HIV |
Author: | Torre Tarazona, Humberto Erick de la González Robles, Alba Cascajero, Almudena Jiménez, Paloma Miró Meda, José M. Sánchez Palomino, Sonsoles Alcamí, José Buzón, María J. García Pérez, Javier Hospital Clinic HIV Investigators |
Keywords: | SARS-CoV-2 Inhibidors de la integrasa VIH (Virus) Neutralitat Persones seropositives SARS-CoV-2 Integrase inhibitors HIV (Viruses) Neutrality HIV-positive persons |
Issue Date: | 2-Feb-2023 |
Publisher: | Wiley |
Abstract: | The presence of neutralizing antibodies (NAbs) is a major correlate of protection for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Thus, different in vitro pseudoviruses-based assays have been described to detect NAbs against SARS-CoV-2. However, the determination of NAbs against SARS-CoV-2 in people living with HIV (PLWH) through HIV-based pseudoparticles could be influenced by cross-neutralization activity or treatment, impeding accurate titration of NAbs. Two assays were compared using replication-defective HIV or VSV-based particles pseudotyped with SARS-CoV-2 spike to measure NAbs in COVID-19-recovered and COVID-19-naïve PLWH. The assay based on HIV-pseudoparticles displayed neutralization activity in all COVID-19-recovered PLWH with a median neutralizing titer 50 (NT50) of 1417.0 (interquartile range [IQR]: 450.3-3284.0), but also in 67% of COVID-19-naïve PLWH (NT50: 631.5, IQR: 16.0-1535.0). Regarding VSV-pseudoparticles system, no neutralization was observed in COVID-19-naïve PLWH as expected, whereas in comparison with HIV-pseudoparticles assay lower neutralization titers were measured in 75% COVID-19-recovered PLWH (NT50: 100.5; IQR: 20.5-1353.0). Treatment with integrase inhibitors was associated with inaccurate increase in neutralization titers when HIV-based pseudoparticles were used. IgG purification and consequent elimination of drugs from samples avoided the interference with retroviral cycle and corrected the lack of specificity observed in HIV-pseudotyped assay. This study shows methodological alternatives based on pseudoviruses systems to determine specific SARS-CoV-2 neutralization titers in PLWH. |
Note: | Reproducció del document publicat a: https://doi.org/10.1002/jmv.28543 |
It is part of: | Journal of Medical Virology, 2023, vol. 95, num. 2 |
URI: | http://hdl.handle.net/2445/196118 |
Related resource: | https://doi.org/10.1002/jmv.28543 |
ISSN: | 0146-6615 |
Appears in Collections: | Articles publicats en revistes (Medicina) Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) |
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