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Title: Substrate adhesion determines migration during mesenchymal cell condensation in chondrogenesis
Author: Casanellas, Ignasi
Jiang, Hongkai
David, Carolyn M.
Vida, Yolanda
Pérez Inestrosa, Ezequiel
Samitier i Martí, Josep
Lagunas, Anna
Keywords: Migració cel·lular
Materials nanoestructurats
Cell migration
Nanostructured materials
Issue Date: 1-Nov-2022
Publisher: The Company of Biologists
Abstract: Mesenchymal condensation is a prevalent morphogenetic transition that is essential in chondrogenesis. However, the current understanding of condensation mechanisms is limited. In vivo, progenitor cells directionally migrate from the surrounding loose mesenchyme towards regions of increasing matrix adherence (the condensation centers), which is accompanied by the upregulation of fibronectin. Here, we focused on the mechanisms of cell migration during mesenchymal cell condensation and the effects of matrix adherence. Dendrimer-based nanopatterns of the cell-adhesive peptide arginine-glycine-aspartic acid (RGD), which is present in fibronectin, were used to regulate substrate adhesion. We recorded collective and single-cell migration of mesenchymal stem cells, under chondrogenic induction, using live-cell imaging. Our results show that the cell migration mode of single cells depends on substrate adhesiveness, and that cell directionality controls cell condensation and the fusion of condensates. Inhibition experiments revealed that cell-cell interactions mediated by N-cadherin (also known as CDH2) are also pivotal for directional migration of cell condensates by maintaining cell-cell cohesion, thus suggesting a fine interplay between cell-matrix and cell-cell adhesions. Our results shed light on the role of cell interactions with a fibronectin-depositing matrix during chondrogenesis in vitro, with possible applications in regenerative medicine. This article has an associated First Person interview with the first author of the paper.
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It is part of: Journal of Cell Science, 2022, vol. 135, num. 22, p. jcs.260241
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ISSN: 0021-9533
Appears in Collections:Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)
Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))

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