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http://hdl.handle.net/2445/197328
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DC Field | Value | Language |
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dc.contributor.author | Pena Rodríguez, Eloy | - |
dc.contributor.author | García Berrocoso, Teresa | - |
dc.contributor.author | Vázquez Fernández, Ezequiel | - |
dc.contributor.author | Otero Espinar, Francisco J. | - |
dc.contributor.author | Abian, Joaquin | - |
dc.contributor.author | Fernández Campos, Francisco | - |
dc.date.accessioned | 2023-04-27T10:55:59Z | - |
dc.date.available | 2023-04-27T10:55:59Z | - |
dc.date.issued | 2023-04-01 | - |
dc.identifier.issn | 1873-3476 | - |
dc.identifier.uri | http://hdl.handle.net/2445/197328 | - |
dc.description.abstract | Two of the most promising techniques in terms of ex vivo skin imaging and quantifying are confocal Raman microscopy and MALDI-TOF mass spectrometry imaging (MALDI-TOF MSI). Both techniques were set up, and the semiquantitative skin biodistribution of previously developed dexamethasone (DEX) loaded lipomers was compared using Benzalkonium chloride (BAK) as a tracer of the nanoparticles. In MALDI-TOF MSI, DEX was derivatised with GirT (DEX-GirT) and the semiquantitative biodistribution of both DEX-GirT and BAK was successfully obtained. The amount of DEX measured by confocal Raman microscopy was higher than that measured by MALDI-TOF MSI, but MALDI-TOF MSI proved to be a more suitable technique for tracing BAK. An absorption-promoting tendency of DEX loaded in lipomers versus a free-DEX solution was observed in confocal Raman microscopy. The higher spatial resolution of confocal Raman microscopy (350 nm) with respect to MALDI-TOF MSI (50 mu m) allowed to observe specific skin structures like hair follicles. Nevertheless, the faster sampling rate of MALDI-TOF-MSI, permitted the analysis of larger tissue regions. In conclusion, both techniques allowed to simultaneously analyze semiquantitative data together with qualitative images of biodistribution, which is a very helpful tool when designing nanoparticles that accumulate in specific anatomical regions. | - |
dc.format.extent | 10 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1016/j.ijpharm.2023.122808 | - |
dc.relation.ispartof | International Journal of Pharmaceutics, 2023, vol. 636 | - |
dc.relation.uri | https://doi.org/10.1016/j.ijpharm.2023.122808 | - |
dc.rights | cc by-nc-nd (c) Pena Rodríguez, Eloy et al, 2023 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.source | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) | - |
dc.subject.classification | Pell | - |
dc.subject.classification | Espectrometria de masses | - |
dc.subject.other | Skin | - |
dc.subject.other | Mass spectrometry | - |
dc.title | Monitoring dexamethasone skin biodistribution with ex vivo MALDI-TOF mass spectrometry imaging and confocal Raman microscopy | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.date.updated | 2023-04-17T13:57:37Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 36889415 | - |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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1-s2.0-S0378517323002284-main.pdf | 6.89 MB | Adobe PDF | View/Open |
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