Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/197363
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dc.contributor.authorEl Ouahabi, Oumaima-
dc.contributor.authorMancera Arteu, Montserrat-
dc.contributor.authorPont Villanueva, Laura-
dc.contributor.authorGiménez López, Estela-
dc.contributor.authorSanz Nebot, María Victoria-
dc.contributor.authorBenavente Moreno, Fernando J. (Julián)-
dc.date.accessioned2023-04-28T17:06:11Z-
dc.date.available2023-04-28T17:06:11Z-
dc.date.issued2022-10-17-
dc.identifier.issn0026-265X-
dc.identifier.urihttp://hdl.handle.net/2445/197363-
dc.description.abstractIn this study, on-line solid-phase extraction capillary liquid chromatography-mass spectrometry (SPE-CapLC-MS) and on-line solid-phase extraction capillary electrophoresis-mass spectrometry (SPE-CE-MS) were compared for the analysis of the opioid peptide biomarkers dynorphin A (1-7) (DynA), endomorphin 1 (End 1), and methionine-enkephalin (Met). First, a capillary liquid chromatography-mass spectrometry (CapLC-MS) method was established, which allowed limits of detection (LODs) of 0.5 μg/mL for Dyn A and Met, and 0.1 μg/mL for End 1. Then, a column switching setup operated by a 2-position/6-port micro-valve with a C18 enrichment column was assembled for SPE-CapLC-MS. Under optimized conditions, the LODs for the three peptides were lowered up to 1000-fold compared to CapLC-MS, until detecting 0.5 ng/mL concentrations. Repeatability (<0.2 % and <11 % RSD for retention times and peak areas, respectively), linearity (0.5-100 ng/mL), and durability (20 runs) of the enrichment column were appropriate, and the method was applied to analyze human plasma samples. Finally, the established SPE-CapLC-MS method was compared with a valve-free C18-SPE-CE-MS method previously described by our group for the analysis of these opioid peptides, using the same mass spectrometer. Both methods presented an evident difference regarding the need of a valve for the operation and allowed high preconcentration factors and quite similar LODs (until 0.5 and 0.1 ng/mL by SPE-CaLC-MS and SPE-CE-MS, respectively). Some other distinctions related to the instrumental set-up, procedure and method performance were also disclosed and discussed in detail.-
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherElsevier B.V.-
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.1016/j.microc.2022.108089-
dc.relation.ispartofMicrochemical Journal, 2022, vol. 183, p. 108089-
dc.relation.urihttps://doi.org/10.1016/j.microc.2022.108089-
dc.rightscc-by-nc-nd (c) El Ouahabi, Oumaima et al., 2022-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.sourceArticles publicats en revistes (Enginyeria Química i Química Analítica)-
dc.subject.classificationElectroforesi capil·lar-
dc.subject.classificationEspectrometria de masses-
dc.subject.classificationNeuropèptids-
dc.subject.otherCapillary electrophoresis-
dc.subject.otherMass spectrometry-
dc.subject.otherNeuropeptides-
dc.titleOn-line solid-phase extraction to enhance sensitivity in peptide biomarker analysis by microseparation techniques coupled to mass spectrometry: capillary liquid chromatography versus capillary electrophoresis-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.identifier.idgrec725100-
dc.date.updated2023-04-28T17:06:11Z-
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess-
Appears in Collections:Articles publicats en revistes (Enginyeria Química i Química Analítica)

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