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Title: Understanding and evolving prions by yeast multiplexed assays
Author: Seuma, Mireia
Bolognesi, Benedetta
Keywords: Prions
Issue Date: 28-Jun-2022
Publisher: Elsevier BV
Abstract: Yeast genetics made it possible to derive the first fundamental insights into prion composition, conformation, and propagation. Fast-forward 30 years and the same model organism is now proving an extremely powerful tool to comprehensively explore the impact of mutations in prion sequences on their function, toxicity, and physical properties. Here, we provide an overview of novel multiplexed strategies where deep mutagenesis is combined to a range of tailored selection assays in yeast, which are particularly amenable for investigating prions and prion-like sequences. By mimicking evolution in a flask, these multiplexed approaches are revealing mechanistic insights on the consequences of prion self-assembly, while also reporting on the structure prion sequences adopt in vivo.
Note: Versió postprint del document publicat a:
It is part of: Current Opinion in Genetics & Development, vol.75, num. 101941
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ISSN: 1879-0380
Appears in Collections:Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))

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