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https://hdl.handle.net/2445/197926| Title: | Implementation of a New Protocol for Direct Identification from Urine in the Routine Microbiological Diagnosis |
| Author: | Zboromyrska, Yuliya Rico, Verónica Pitart, Cristina Fernandez Pittol, Mariana José Soriano,Àlex Bosch Mestres, Jordi |
| Keywords: | Infeccions del tracte urinari Bacteris patògens Urinary tract infections Pathogenic bacteria |
| Issue Date: | 26-Apr-2022 |
| Abstract: | The direct identification of uropathogens from urine samples, in combination with the rapid detection of resistance, would allow early adjustment of empirical antimicrobial treatment.Two hundred and ninety-eight urine samples processed between 1 June and 31 December 2020, selected with flow cytometry, with direct identification by MALDI-TOF mass spectrometry, and rapid detection of extended-spectrum beta-lactamase (ESBL) and carbapenemases-producing strains by lateral flow were analyzed.The positive predictive value of the direct identification of the 86 samples that met the flow cytometry criterion (>5000 bacteria/µL) was 96.4%. Reliable direct identification was obtained in 14 of the 27 (51.8%) urinary source bacteraemias. There was 100% agreement between the lateral flow and antibiogram in the detection of ESBL and carbapenemases.the protocol for the direct identification and rapid detection of ESBL and carbapenemases-producing strains from urine samples is a reliable and useful tool. |
| Note: | Reproducció del document publicat a: https://doi.org/10.3390/antibiotics11050582 |
| It is part of: | Antibiotics, 2022, vol. 11, num. 5 |
| URI: | https://hdl.handle.net/2445/197926 |
| Related resource: | https://doi.org/10.3390/antibiotics11050582 |
| ISSN: | 2079-6382 |
| Appears in Collections: | Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| Implementation of a New Protocol for Direct Identification_Antibiotics.pdf | 429.7 kB | Adobe PDF | View/Open |
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