Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/197926
Title: Implementation of a New Protocol for Direct Identification from Urine in the Routine Microbiological Diagnosis
Author: Zboromyrska, Yuliya
Rico, Verónica
Pitart, Cristina
Fernandez Pittol, Mariana José
Soriano,Àlex
Bosch Mestres, Jordi
Keywords: Infeccions del tracte urinari
Bacteris patògens
Urinary tract infections
Pathogenic bacteria
Issue Date: 26-Apr-2022
Abstract: The direct identification of uropathogens from urine samples, in combination with the rapid detection of resistance, would allow early adjustment of empirical antimicrobial treatment.Two hundred and ninety-eight urine samples processed between 1 June and 31 December 2020, selected with flow cytometry, with direct identification by MALDI-TOF mass spectrometry, and rapid detection of extended-spectrum beta-lactamase (ESBL) and carbapenemases-producing strains by lateral flow were analyzed.The positive predictive value of the direct identification of the 86 samples that met the flow cytometry criterion (>5000 bacteria/µL) was 96.4%. Reliable direct identification was obtained in 14 of the 27 (51.8%) urinary source bacteraemias. There was 100% agreement between the lateral flow and antibiogram in the detection of ESBL and carbapenemases.the protocol for the direct identification and rapid detection of ESBL and carbapenemases-producing strains from urine samples is a reliable and useful tool.
Note: Reproducció del document publicat a: https://doi.org/10.3390/antibiotics11050582
It is part of: Antibiotics, 2022, vol. 11, num. 5
URI: http://hdl.handle.net/2445/197926
Related resource: https://doi.org/10.3390/antibiotics11050582
ISSN: 2079-6382
Appears in Collections:Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)

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