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Title: On the uptake of cationic liposomes by cells: from changes in elasticity to internalization
Author: Botet Carreras, Adrià
Bosch Marimon, Manel
Millan Solsona, Ruben
Aubets Gil, Eva
Ciudad i Gómez, Carlos Julián
Noé Mata, Verónica
Montero, M.Teresa
Domènech Cabrera, Òscar
Borrell Hernández, Jordi
Keywords: Liposomes
Sistemes d'alliberament de medicaments
Microscòpia de força atòmica
Drug delivery systems
Atomic force microscopy
Issue Date: 1-Jan-2023
Publisher: Elsevier B.V.
Abstract: In this study, we assessed the capacity of a previously reported engineered liposomal formulation, which had been tested against model membranes mimicking the lipid composition of the HeLa plasma membrane, to fuse and function as a nanocarrier in cells. We used atomic force microscopy to observe physicochemical changes on the cell surface and confocal microscopy to determine how the liposomes interact with cell membranes and released their load. In addition, we performed viability assays using methotrexate as an active drug to obtain proof of concept of the formulation´s capacity to function as a drug delivery-system. The interaction of engineered liposomes with living cells corroborates the information obtained using model membranes and supports the capacity of the engineered liposomal formulation to serve as a potential nanocarrier.
Note: Reproducció del document publicat a:
It is part of: Colloids and Surfaces B-Biointerfaces, 2023, vol. 221, p. 112968
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ISSN: 0927-7765
Appears in Collections:Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)
Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
Articles publicats en revistes (Centres Científics i Tecnològics de la Universitat de Barcelona (CCiTUB))
Articles publicats en revistes (Bioquímica i Fisiologia)
Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
Articles publicats en revistes (Farmàcia, Tecnologia Farmacèutica i Fisicoquímica)
Articles publicats en revistes (Institut de Nanociència i Nanotecnologia (IN2UB))

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