Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/198767
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dc.contributor.authorComelles Pujadas, Jordi-
dc.contributor.authorFernández Majada, Vanesa-
dc.contributor.authorAcevedo, Verónica-
dc.contributor.authorRebollo Calderon, Beatriz-
dc.contributor.authorMartínez Fraiz, Elena-
dc.date.accessioned2023-06-01T11:18:04Z-
dc.date.available2023-06-01T11:18:04Z-
dc.date.issued2022-01-27-
dc.identifier.issn2590-0064-
dc.identifier.urihttp://hdl.handle.net/2445/198767-
dc.description.abstractTopographical patterns are a powerful tool to study directional migration. Grooved substrates have been extensively used as in vitro models of aligned extracellular matrix fibers because they induce cell elongation, alignment, and migration through a phenomenon known as contact guidance. This process, which involves the orientation of focal adhesions, F-actin, and microtubule cytoskeleton along the direction of the grooves, has been primarily studied on hard materials of non-physiological stiffness. But how it unfolds when the stiffness of the grooves varies within the physiological range is less known. Here we show that substrate stiffness modulates the cellular response to topographical contact guidance. We find that for fibroblasts, while focal adhesions and actin respond to topography independently of the stiffness, microtubules show a stiffness-dependent response that regulates contact guidance. On the other hand, both clusters and single breast carcinoma epithelial cells display stiffnessdependent contact guidance, leading to more directional and efficient migration when increasing substrate stiffness. These results suggest that both matrix stiffening and alignment of extracellular matrix fibers cooperate during directional cell migration, and that the outcome differs between cell types depending on how they organize their cytoskeletons.-
dc.format.extent15 p.-
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherElsevier-
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.1016/j.mtbio.2023.100593-
dc.relation.ispartofMaterials Today Bio, 2022, vol. 19, num. 100593-
dc.relation.urihttps://doi.org/10.1016/j.mtbio.2023.100593-
dc.rightscc-by (c) Comelles, Jordi et al., 2022-
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/-
dc.sourceArticles publicats en revistes (Enginyeria Electrònica i Biomèdica)-
dc.subject.classificationMigració cel·lular-
dc.subject.classificationTopografia-
dc.subject.classificationCitosquelet-
dc.subject.classificationFibroblasts-
dc.subject.otherCell migration-
dc.subject.otherTopography-
dc.subject.otherCytoskeleton-
dc.subject.otherFibroblasts-
dc.titleSoft topographical patterns trigger a stiffness-dependent cellular response to contact guidance-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.identifier.idgrec722260-
dc.date.updated2023-06-01T11:18:04Z-
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess-
dc.identifier.pmid36923364-
Appears in Collections:Articles publicats en revistes (Patologia i Terapèutica Experimental)
Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)
Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))

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