Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/200290
Title: | Ratiometric Nanothermometer Based on a Radical Excimer for In Vivo Sensing |
Author: | Blasi, Davide González Pato, Nerea Rodríguez Rodríguez, Xavier Díez Zabala, Iñigo Srinivasan, Sumithra Yasaswini Camarero, Núria Esquivias, Oriol Roldan, Mònica Guasch, Judith Laromaine, Anna Gorostiza, Pau Veciana, Jaume Ratera, Imma |
Keywords: | Cèl·lules Nanomedicina Cells Nanomedicine |
Issue Date: | 14-Apr-2023 |
Publisher: | Wiley |
Abstract: | Ratiometric fluorescent nanothermometers with near-infrared emission play an important role in in vivo sensing since they can be used as intracellular thermal sensing probes with high spatial resolution and high sensitivity, to investigate cellular functions of interest in diagnosis and therapy, where current approaches are not effective. Herein, the temperature-dependent fluorescence of organic nanoparticles is designed, synthesized, and studied based on the dual emission, generated by monomer and excimer species, of the tris(2,4,6-trichlorophenyl)methyl radical (TTM) doping organic nanoparticles (TTMd-ONPs), made of optically neutral tris(2,4,6-trichlorophenyl)methane (TTM-αH), acting as a matrix. The excimer emission intensity of TTMd-ONPs decreases with increasing temperatures whereas the monomer emission is almost independent and can be used as an internal reference. TTMd-ONPs show a great temperature sensitivity (3.4% K-1 at 328 K) and a wide temperature response at ambient conditions with excellent reversibility and high colloidal stability. In addition, TTMd-ONPs are not cytotoxic and their ratiometric outputs are unaffected by changes in the environment. Individual TTMd-ONPs are able to sense temperature changes at the nano-microscale. In vivo thermometry experiments in Caenorhabditis elegans (C. elegans) worms show that TTMd-ONPs can locally monitor internal body temperature changes with spatio-temporal resolution and high sensitivity, offering multiple applications in the biological nanothermometry field.© 2023 The Authors. Small published by Wiley-VCH GmbH. |
Note: | https://doi.org/10.1002/smll.202207806 |
It is part of: | Small, 2023, 2207806-NA |
URI: | http://hdl.handle.net/2445/200290 |
Related resource: | https://doi.org/10.1002/smll.202207806 |
ISSN: | 1613-6829 |
Appears in Collections: | Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
2023_Small_Ratiometric_BlasiD.pdf | 1.72 MB | Adobe PDF | View/Open |
This item is licensed under a
Creative Commons License