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http://hdl.handle.net/2445/201181
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DC Field | Value | Language |
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dc.contributor.author | Dotti, Isabella | - |
dc.contributor.author | Mayorgas, Aida | - |
dc.contributor.author | Salas, Azucena | - |
dc.date.accessioned | 2023-07-26T08:07:46Z | - |
dc.date.available | 2023-07-26T08:07:46Z | - |
dc.date.issued | 2022-10-27 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/2445/201181 | - |
dc.description.abstract | Ulcerative colitis and Crohn's disease are chronic inflammatory bowel diseases (IBD) of unknown cause characterized by a relapsing-remitting behavior. Growing evidence supports the idea that the epithelial barrier plays a central role in the pathogenesis of IBD as well as in its evolution over time, thus representing a potential target for novel therapeutic options. In the last decade, the introduction of 3D epithelial cultures from ex vivo-expanded intestinal adult stem cells (ASCs) has impacted our ability to study the function of the epithelium in several gastrointestinal disorders, including IBD. Here, we describe in detail a reproducible protocol to generate Matrigel-embedded epithelial organoids from ASCs of non-IBD and IBD donors using small colonic biopsies, including steps for its optimization. A slightly modified version of this protocol is also provided in case surgical samples are used. With this method, epithelial organoids can be expanded over several passages, thereby generating a large quantity of viable cells that can be used in multiple downstream analyses including genetic, transcriptional, proteomic and/or functional studies. In addition, 3D cultures generated using our protocol are suitable for the establishment of 2D cultures, which can model relevant cell-to-cell interactions that occur in IBD mucosa. | - |
dc.format.extent | 13 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0276195 | - |
dc.relation.ispartof | Plos One, 2022, vol. 17, num. 10 | - |
dc.relation.uri | https://doi.org/10.1371/journal.pone.0276195 | - |
dc.rights | cc by (c) Dotti, Isabella et al, 2022 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.source | Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) | - |
dc.subject.classification | Malalties inflamatòries intestinals | - |
dc.subject.classification | Membrana mucosa | - |
dc.subject.other | Inflammatory bowel diseases | - |
dc.subject.other | Mucous membrane | - |
dc.title | Generation of human colon organoids from healthy and inflammatory bowel disease mucosa | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.date.updated | 2023-07-10T07:50:53Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.idimarina | 9331502 | - |
dc.identifier.pmid | 36301950 | - |
Appears in Collections: | Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) |
Files in This Item:
File | Description | Size | Format | |
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Generation of human colon organoids from healthy and inflammatory bowel disease mucosa_PlosOne.pdf | 2.7 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License