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http://hdl.handle.net/2445/201835
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DC Field | Value | Language |
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dc.contributor.author | Arpa Toribio, Luis | - |
dc.contributor.author | Batlle, Carlos | - |
dc.contributor.author | Jiang, Peijin | - |
dc.contributor.author | Caelles Franch, Carme | - |
dc.contributor.author | Lloberas Cavero, Jorge | - |
dc.contributor.author | Celada Cotarelo, Antonio | - |
dc.date.accessioned | 2023-09-08T11:36:28Z | - |
dc.date.available | 2023-09-08T11:36:28Z | - |
dc.date.issued | 2023-04-11 | - |
dc.identifier.issn | 2073-4409 | - |
dc.identifier.uri | http://hdl.handle.net/2445/201835 | - |
dc.description.abstract | IL(Interleukin)-4 is the main macrophage M2-type activator and induces an anti-inflammatory phenotype called alternative activation. The IL-4 signaling pathway involves the activation of STAT (Signal Transducer and Activator of Transcription)-6 and members of the MAPK (Mitogen-activated protein kinase) family. In primary-bone-marrow-derived macrophages, we observed a strong activation of JNK (Jun N-terminal kinase)-1 at early time points of IL-4 stimulation. Using selective inhibitors and a knockout model, we explored the contribution of JNK-1 activation to macrophages' response to IL-4. Our findings indicate that JNK-1 regulates the IL-4-mediated expression of genes typically involved in alternative activation, such as Arginase 1 or Mannose receptor, but not others, such as SOCS (suppressor of cytokine signaling) 1 or p21Waf−1 (cyclin dependent kinase inhibitor 1A). Interestingly, we have observed that after macrophages are stimulated with IL-4, JNK-1 has the capacity to phosphorylate STAT-6 on serine but not on tyrosine. Chromatin immunoprecipitation assays revealed that functional JNK-1 is required for the recruitment of co-activators such as CBP (CREB-binding protein)/p300 on the promoter of Arginase 1 but not on p21Waf−1. Taken together, these data demonstrate the critical role of STAT-6 serine phosphorylation by JNK-1 in distinct macrophage responses to IL-4. | - |
dc.format.extent | 18 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | MDPI | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.3390/cells12081127 | - |
dc.relation.ispartof | Cells, 2023, vol. 12, num. 8, p. 1127-1144 | - |
dc.relation.uri | https://doi.org/10.3390/cells12081127 | - |
dc.rights | cc-by (c) Arpa Toribio, Luis et al., 2023 | - |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.source | Articles publicats en revistes (Bioquímica i Fisiologia) | - |
dc.subject.classification | Macròfags | - |
dc.subject.classification | Inflamació | - |
dc.subject.other | Macrophages | - |
dc.subject.other | Inflammation | - |
dc.title | Distinct Responses to IL4 in Macrophages Mediated by JNK | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 733339 | - |
dc.date.updated | 2023-09-08T11:36:28Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) Articles publicats en revistes (Bioquímica i Fisiologia) |
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733339.pdf | 3.17 MB | Adobe PDF | View/Open |
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