Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/202059
Títol: Newly designed poxviral promoters to improve immunogenicity and efficacy of MVA-NP candidate vaccine against lethal influenza virus infection in mice
Autor: Langenmayer, Martin C.
Luelf-Averhoff, Anna-Theresa
Marr, Lisa
Jany, Sylvia
Freudenstein, Astrid
Adam-Neumair, Silvia
Tscherne, Alisa
Fux, Robert
Rojas, Juan José
Blutke, Andreas
Sutter, Gerd
Volz, Asisa
Matèria: Influenzavirus
Nucleoproteïnes
Vacunes
Ratolins (Animals de laboratori)
Influenza viruses
Nucleoproteins
Vaccines
Mice (Laboratory animals)
Data de publicació: 23-juny-2023
Publicat per: MDPI
Resum: Influenza, a respiratory disease mainly caused by influenza A and B, viruses of the Orthomyxoviridae, is still a burden on our society's health and economic system. Influenza A viruses (IAV) circulate in mammalian and avian populations, causing seasonal outbreaks with high numbers of cases. Due to the high variability in seasonal IAV triggered by antigenic drift, annual vaccination is necessary, highlighting the need for a more broadly protective vaccine against IAV. The safety tested Modified Vaccinia virus Ankara (MVA) is licensed as a third-generation vaccine against smallpox and serves as a potent vector system for the development of new candidate vaccines against different pathogens. Here, we generated and characterized recombinant MVA candidate vaccines that deliver the highly conserved internal nucleoprotein (NP) of IAV under the transcriptional control of five newly designed chimeric poxviral promoters to further increase the immunogenic properties of the recombinant viruses (MVA-NP). Infections of avian cell cultures with the recombinant MVA-NPs demonstrated efficient synthesis of the IAV-NP which was expressed under the control of the five new promoters. Prime-boost or single shot immunizations in C57BL/6 mice readily induced circulating serum antibodies' binding to recombinant IAV-NP and the robust activation of IAV-NP-specific CD8+ T cell responses. Moreover, the MVA-NP candidate vaccines protected C57BL/6 mice against lethal respiratory infection with mouse-adapted IAV (A/Puerto Rico/8/1934/H1N1). Thus, further studies are warranted to evaluate the immunogenicity and efficacy of these recombinant MVA-NP vaccines in other IAV challenge models in more detail.
Nota: Reproducció del document publicat a: https://doi.org/10.3390/pathogens12070867
És part de: Pathogens, 2023, vol. 12, num. 7
URI: https://hdl.handle.net/2445/202059
Recurs relacionat: https://doi.org/10.3390/pathogens12070867
ISSN: 2076-0817
Apareix en les col·leccions:Articles publicats en revistes (Patologia i Terapèutica Experimental)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))

Fitxers d'aquest document:
Fitxer Descripció DimensionsFormat 
738499.pdf5.35 MBAdobe PDFMostrar/Obrir


Aquest document està subjecte a una Llicència Creative Commons Creative Commons