Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/205222
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Pedraz López, Lucas | - |
dc.contributor.author | Torrents Serra, Eduard | - |
dc.date.accessioned | 2024-01-03T18:14:00Z | - |
dc.date.available | 2024-01-03T18:14:00Z | - |
dc.date.issued | 2023-10-26 | - |
dc.identifier.issn | 1940-9818 | - |
dc.identifier.uri | http://hdl.handle.net/2445/205222 | - |
dc.description.abstract | Fluorescent proteins, such as green fluorescent proteins, are invaluable tools for detecting and quantifying gene expression in high-throughput reporter gene assays. However, they introduce significant inaccuracies in studies involving microaerobiosis or anaerobiosis, as oxygen is required for the maturation of these proteins' chromophores. In this study, the authors highlight the errors incurred by using fluorescent proteins under limited oxygenation by comparing standard fluorescence-based reporter gene assays to quantitative real-time PCR data in the study of a complex oxygen-regulated gene network. Furthermore, a solution to perform quantification of anaerobic and microaerobic gene expression with fluorescent reporter proteins using a microplate reader with an oxygen control system and applying pulses of full oxygenation before fluorescence measurements is provided. | - |
dc.format.extent | 6 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Future Science | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.2144/btn-2023-0064 | - |
dc.relation.ispartof | Biotechniques, 2023, vol. 75, num. 6, p. 250-255 | - |
dc.relation.uri | https://doi.org/10.2144/btn-2023-0064 | - |
dc.rights | cc by-nc-nd (c) Pedraz López, Lucas et al, 2023 | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | * |
dc.source | Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) | - |
dc.subject.classification | Bioluminescència | - |
dc.subject.classification | Expressió gènica | - |
dc.subject.other | Bioluminescence | - |
dc.subject.other | Gene expression | - |
dc.title | An easy method for quantification of anaerobic and microaerobic gene expression with fluorescent reporter proteins | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.date.updated | 2023-12-19T09:15:57Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.idimarina | 6605019 | - |
dc.identifier.pmid | 37880975 | - |
Appears in Collections: | Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
2023_FutSci_AnEasy_TorrentsE.pdf | 356.23 kB | Adobe PDF | View/Open |
This item is licensed under a
Creative Commons License