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Title: Proton and alpha radiation-induced mutational profiles in human cells
Author: Delhomme, T. M.
Munteanu, M.
Buonanno, M.
Grilj, V.
Biayna, J.
Supek, F.
Keywords: Mutació (Biologia)
Radiació ionitzant
Mutation (Biology)
Ionizing radiation
Issue Date: 16-Jun-2023
Publisher: Nature Research
Abstract: Ionizing radiation is known to be DNA damaging and mutagenic, however less is known about which mutational footprints result from exposures of human cells to different types of radiation. We were interested in the mutagenic effects of particle radiation exposures on genomes of various human cell types, in order to gauge the genotoxic risks of galactic cosmic radiation, and of certain types of tumor radiotherapy. To this end, we exposed cultured cell lines from the human blood, breast and lung to fractionated proton and alpha particle (helium nuclei) beams at doses sufficient to considerably affect cell viability. Whole-genome sequencing revealed that mutation rates were not overall markedly increased upon proton and alpha exposures. However, there were modest changes in mutation spectra and distributions, such as the increases in clustered mutations and of certain types of indels and structural variants. The spectrum of mutagenic effects of particle beams may be cell-type and/or genetic background specific. Overall, the mutational effects of repeated exposures to proton and alpha radiation on human cells in culture appear subtle, however further work is warranted to understand effects of long-term exposures on various human tissues.© 2023. The Author(s).
Note: Reproducció del document publicat a:
It is part of: Scientific Reports, 2023, vol. 13, num. 1, p. 9791
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ISSN: 2045-2322
Appears in Collections:Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))

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