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http://hdl.handle.net/2445/206796
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DC Field | Value | Language |
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dc.contributor.author | Lipstein, Noa | - |
dc.contributor.author | Chang, Shuwen | - |
dc.contributor.author | Lin, Kun-Han | - |
dc.contributor.author | López Murcia, Francisco José | - |
dc.contributor.author | Neher, Erwin | - |
dc.contributor.author | Taschenberger, Holger | - |
dc.contributor.author | Brose, Nils | - |
dc.date.accessioned | 2024-01-31T15:21:25Z | - |
dc.date.available | 2024-01-31T15:21:25Z | - |
dc.date.issued | 2021-12 | - |
dc.identifier.issn | 0896-6273 | - |
dc.identifier.uri | http://hdl.handle.net/2445/206796 | - |
dc.description.abstract | During ongoing presynaptic action potential (AP) firing, transmitter release is limited by the availability of release-ready synaptic vesicles (SVs). The rate of SV recruitment (SVR) to release sites is strongly upregulated at high AP frequencies to balance SV consumption. We show that Munc13-1-an essential SV priming protein-regulates SVR via a Ca2+-phospholipid-dependent mechanism. Using knockin mouse lines with point mutations in the Ca2+-phospholipid-binding C2B domain of Munc13-1, we demonstrate that abolishing Ca2+-phospholipid binding increases synaptic depression, slows recovery of synaptic strength after SV pool depletion, and reduces temporal fidelity of synaptic transmission, while increased Ca2+-phospholipid binding has the opposite effects. Thus, Ca2+-phospholipid binding to the Munc13-1-C2B domain accelerates SVR, reduces short-term synaptic depression, and increases the endurance and temporal fidelity of neurotransmission, demonstrating that Munc13-1 is a core vesicle priming hub that adjusts SV re-supply to demand. | - |
dc.format.extent | 29 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Cell Press | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1016/j.neuron.2021.09.054 | - |
dc.relation.ispartof | Neuron, 2021, vol. 109, num.24, p. 3980-4000.e7 | - |
dc.relation.uri | https://doi.org/10.1016/j.neuron.2021.09.054 | - |
dc.rights | cc-by-nc-nd (c) Lipstein, Noa et al, 2021 | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
dc.source | Articles publicats en revistes (Patologia i Terapèutica Experimental) | - |
dc.subject.classification | Sinapsi | - |
dc.subject.classification | Neurotransmissió | - |
dc.subject.classification | Neuroplasticitat | - |
dc.subject.other | Synapses | - |
dc.subject.other | Neural transmission | - |
dc.subject.other | Neuroplasticity | - |
dc.title | Munc13-1 is a Ca2+-phospholipid-dependent vesicle priming hub that shapes synaptic short-term plasticity and enables sustained neurotransmission | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 725955 | - |
dc.date.updated | 2024-01-31T15:21:25Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 34706220 | - |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) |
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252993.pdf | 9.31 MB | Adobe PDF | View/Open |
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