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http://hdl.handle.net/2445/207292
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DC Field | Value | Language |
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dc.contributor.author | Pizarek, John A. | - |
dc.contributor.author | Fischer, Nicholas G. | - |
dc.contributor.author | Aparicio, Conrado | - |
dc.date.accessioned | 2024-02-07T19:34:20Z | - |
dc.date.available | 2024-02-07T19:34:20Z | - |
dc.date.issued | 2023-01-13 | - |
dc.identifier.citation | Pizarek, John A;Fischer, Nicholas G;Aparicio, Conrado. Immunomodulatory IL-23 receptor antagonist peptide nanocoatings for implant soft tissue healing. Dental Materials, 2023, 39, 2, 204-216 | - |
dc.identifier.issn | 0109-5641 | - |
dc.identifier.uri | http://hdl.handle.net/2445/207292 | - |
dc.description.abstract | Peri-implantitis, caused by an inflammatory response to pathogens, is the leading cause of dental implant failure. Poor soft tissue healing surrounding implants - caused by inadequate surface properties - leads to infection, inflammation, and dysregulated keratinocyte and macrophage function. One activated inflammatory response, active around peri-implantitis compared to healthy sites, is the IL-23/IL-17A cytokine axis. Implant surfaces can be synthesized with peptide nanocoatings to present immunomodulatory motifs to target peri-implant keratinocytes to control macrophage polarization and regulate inflammatory axises toward enhancing soft tissue healing.We synthesized an IL-23 receptor (IL-23R) noncompetitive antagonist peptide nanocoating using silanization and evaluated keratinocyte secretome changes and macrophage polarization (M1-like "pro-inflammatory" vs. M2-like "pro-regenerative").IL-23R antagonist peptide nanocoatings were successfully synthesized on titanium, to model dental implant surfaces, and compared to nonfunctional nanocoatings and non-coated titanium. IL-23R antagonist nanocoatings significantly decreased keratinocyte IL-23, and downstream IL-17A, expression compared to controls. This peptide noncompetitive antagonistic function was demonstrated under lipopolysaccharide stimulation. Large scale changes in keratinocyte secretome content, toward a pro-regenerative milieu, were observed from keratinocytes cultured on the IL-23R antagonist nanocoatings compared to controls. Conditioned medium collected from keratinocytes cultured on the IL-23R antagonist nanocoatings polarized macrophages toward a M2-like phenotype, based on increased CD163 and CD206 expression and reduced iNOS expression, compared to controls.Our results support development of IL-23R noncompetitive antagonist nanocoatings to reduce the pro-inflammatory IL-23/17A pathway and augment macrophage polarization toward a pro-regenerative phenotype. Immunomodulatory implant surface engineering may promote soft tissue healing and thereby reduce rates of peri-implantitis.Copyright © 2023 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 29 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier B. V. | - |
dc.relation.isformatof | https://doi.org/10.1016/j.dental.2023.01.001 | - |
dc.relation.ispartof | Dental Materials, 2023, vol. 39, num. 2, p. 204-216 | - |
dc.relation.uri | https://doi.org/10.1016/j.dental.2023.01.001 | - |
dc.rights | cc by-nc-nd (c) The Academy of Dental Materials, 2023 | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | * |
dc.source | Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) | - |
dc.subject.classification | Implants dentals | - |
dc.subject.classification | Interleucines | - |
dc.subject.other | Dental implants | - |
dc.subject.other | Interleukins | - |
dc.title | Immunomodulatory IL-23 receptor antagonist peptide nanocoatings for implant soft tissue healing | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.date.updated | 2024-01-31T10:56:29Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.idimarina | 6574044 | - |
dc.identifier.pmid | 36642687 | - |
Appears in Collections: | Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) |
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File | Description | Size | Format | |
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2023_DentMat_Immunomodulatory_AparicioC_postprint.pdf | 1.27 MB | Adobe PDF | View/Open |
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