Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/207943
Title: | Serum methylation of GALNT9, UPF3A, WARS, and LDB2 as noninvasive biomarkers for the early detection of colorectal cancer and advanced adenomas |
Author: | Gallardo Gómez, María Rodríguez Girondo, Mar Planell, Núria Moran, Sebastian Bujanda, Luis Etxart, Ane Castells Garangou, Antoni Balaguer, Francesc Jover, Rodrigo Esteller, Manel Cubiella, Joaquín Gómez Cabrero, David De Chiara, Loretta |
Keywords: | RNA Càncer RNA Cancer |
Issue Date: | 4-Oct-2023 |
Publisher: | Springer Science and Business Media LLC |
Abstract: | Background Early detection has proven to be the most effective strategy to reduce the incidence and mortality of colorectal cancer (CRC). Nevertheless, most current screening programs suffer from low participation rates. A blood test may improve both the adherence to screening and the selection to colonoscopy. In this study, we conducted a serum-based discovery and validation of cfDNA methylation biomarkers for CRC screening in a multicenter cohort of 433 serum samples including healthy controls, benign pathologies, advanced adenomas (AA), and CRC.Results First, we performed an epigenome-wide methylation analysis with the MethylationEPIC array using a sample pooling approach, followed by a robust prioritization of candidate biomarkers for the detection of advanced neoplasia (AN: AA and CRC). Then, candidate biomarkers were validated by pyrosequencing in independent individual cfDNA samples. We report GALNT9, UPF3A, WARS, and LDB2 as new noninvasive biomarkers for the early detection of AN. The combination of GALNT9/UPF3A by logistic regression discriminated AN with 78.8% sensitivity and 100% specificity, outperforming the commonly used fecal immunochemical test and the methylated SEPT9 blood test.Conclusions Overall, this study highlights the utility of cfDNA methylation for CRC screening. Our results suggest that the combination methylated GALNT9/UPF3A has the potential to serve as a highly specific and sensitive blood-based test for screening and early detection of CRC. |
Note: | Reproducció del document publicat a: https://doi.org/10.1186/s13148-023-01570-1 |
It is part of: | Clinical Epigenetics, 2023, vol. 15, num. 1 |
URI: | http://hdl.handle.net/2445/207943 |
Related resource: | https://doi.org/10.1186/s13148-023-01570-1 |
ISSN: | 1868-7083 |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
s13148-023-01570-1.pdf | 4.84 MB | Adobe PDF | View/Open |
This item is licensed under a
Creative Commons License