Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/208053
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Martín Venegas, Raquel | - |
dc.contributor.author | Rodríguez Lagunas, María José | - |
dc.contributor.author | Mercier, Yves | - |
dc.contributor.author | Geraert, Pierre-André | - |
dc.contributor.author | Ferrer i Roig, Ruth | - |
dc.date.accessioned | 2024-02-26T12:02:11Z | - |
dc.date.available | 2024-02-26T12:02:11Z | - |
dc.date.issued | 2009-03-01 | - |
dc.identifier.issn | 0363-6143 | - |
dc.identifier.uri | http://hdl.handle.net/2445/208053 | - |
dc.description.abstract | The transport systems involved in intestinal methionine (Met) absorption are described as Na(+)-dependent and Na(+)-independent mechanisms. However, since recent studies have suggested the importance of the H(+) gradient as a driving force for intestinal nutrient absorption, the aim of the present work was to test whether Met transport across the apical membrane of Caco-2 cells is affected by extracellular pH. The results show that l- and d-Met uptake was increased by lowering extracellular pH from 7.4 to 5.5, in both the presence and absence of Na(+). Cis-inhibition experiments revealed that inhibition of l-Met transport by 2-aminobicyclo[2,2,1]heptane-2-carboxylic acid (BCH) or l-lysine (l-Lys) was higher at a pH of 5.5. Moreover, the BCH-insensitive component was not affected by pH, whereas the l-Lys-insensitive component was increased by lowering extracellular pH, thus suggesting the participation of system L. The contribution of another mechanism, sensitive to both BCH and l-Lys, was also considered. The inhibition obtained with taurine (Tau) was also higher at a pH of 5.5, thus suggesting the involvement of system B(0,+) on pH-stimulated component. As for d-Met uptake, the results showed higher inhibition with l-Lys and Tau at a pH of 5.5 and no effect on the l-Lys- or Tau-insensitive component. In conclusion, Met transport across the apical membrane of Caco-2 cells is increased by low extracellular pH as the result of the stimulation of two transport systems functionally identified with systems L and B(0,+) for l-Met and with system B(0,+) for d-Met. | - |
dc.format.extent | 7 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Physiological Society | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1152/ajpcell.00478.2008 | - |
dc.relation.ispartof | American Journal of Physiology-Cell Physiology, 2009, vol. 296, num.3, p. C632-C638 | - |
dc.relation.uri | https://doi.org/10.1152/ajpcell.00478.2008 | - |
dc.rights | (c) American Physiological Society, 2009 | - |
dc.source | Articles publicats en revistes (Bioquímica i Fisiologia) | - |
dc.subject.classification | Membranes cel·lulars | - |
dc.subject.classification | Absorció intestinal | - |
dc.subject.classification | Intestins | - |
dc.subject.other | Cell membranes | - |
dc.subject.other | Intestinal absorption | - |
dc.subject.other | Intestines | - |
dc.title | Effect of pH on L- and D-methionine uptake across the apical membrane of Caco-2 cells | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 696464 | - |
dc.date.updated | 2024-02-26T12:02:12Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Fisiologia) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
224429.pdf | 959.33 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.