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Title: | CD73 controls Myosin II–driven invasion, metastasis, and immunosuppression in amoeboid pancreatic cancer cells |
Author: | Samain, Remi Maiques, Oscar Monger, Joanne Lam, Hoyin Candido, Juliana George, Samantha Ferrari, Nicola Kohihammer, Leonie Lunetto, Sophia Varela, Adrian Orgaz, Jose L. Vilardell, Felip Olsina, Jorge Juan Matias Guiu, Xavier Sarker, Debashis Biddle, Adrian Balkwill, Frances R. Eyles, Jim Wilkinson, Robert W. Kocher, Hemant M. Calvo, Fernando Wells, Claire M. Sanz Moreno, Victoria |
Keywords: | Amebes Proteïnes citosquelètiques Amoeba Cytoskeletal Proteins |
Issue Date: | 20-Oct-2023 |
Publisher: | American Association for the Advancement of Science (AAAS) |
Abstract: | Pancreatic ductal adenocarcinoma (PDAC) has a very poor prognosis because of its high propensity to metastasize and its immunosuppressive microenvironment. Using a panel of pancreatic cancer cell lines, three-dimensional (3D) invasion systems, microarray gene signatures, microfluidic devices, mouse models, and intravital imaging, we demonstrate that ROCK-Myosin II activity in PDAC cells supports a transcriptional program conferring amoeboid invasive and immunosuppressive traits and in vivo metastatic abilities. Moreover, we find that immune checkpoint CD73 is highly expressed in amoeboid PDAC cells and drives their invasive, metastatic, and immunomodulatory traits. Mechanistically, CD73 activates RhoA-ROCK-Myosin II downstream of PI3K. Tissue microarrays of human PDAC biopsies combined with bioinformatic analysis reveal that rounded-amoeboid invasive cells with high CD73-ROCK-Myosin II activity and their immunosuppressive microenvironment confer poor prognosis to patients. We propose targeting amoeboid PDAC cells as a therapeutic strategy. |
Note: | Reproducció del document publicat a: https://doi.org/10.1126/sciadv.adi0244 |
It is part of: | Science Advances, 2023, vol. 9, num. 42 |
URI: | http://hdl.handle.net/2445/208708 |
Related resource: | https://doi.org/10.1126/sciadv.adi0244 |
ISSN: | 2375-2548 |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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