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http://hdl.handle.net/2445/209208| Title: | Analysis of autophagic vesicles in mitotic cells |
| Author: | Almacellas i Canals, Eugènia García Cajide, Marta Tauler Girona, Albert Mauvezin, Caroline |
| Keywords: | Lisosomes Autofàgia Lysosomes Autophagy |
| Issue Date: | 1-Jan-2022 |
| Abstract: | The detection of autophagic vesicles in interphase cells is well characterized with markers such as LC3, SQSTM1 (also known as p62) and LAMP2, which are commonly used in immunofluorescence and biochemistry assays to evaluate the status of autophagy in adherent cells. During mitosis, cells undergo important morphological changes which alter the position of the central plane, therefore the imaging on dividing cells has to be specifically designed. Here, we describe a method to label and image autophagic vesicles in mitotic cells to systematically analyze their number, morphology and distribution. |
| Note: | Versió postprint del capítol del llibre publicat a: https://doi.org/10.1007/978-1-0716-2071-7_9 |
| It is part of: | Autophagy and Cancer. Methods in Molecular Biology, vol 2445, Norberg, H., Norberg, E. (eds), ISBN: 978-1-0716-2071-7, p. 127-137 |
| URI: | http://hdl.handle.net/2445/209208 |
| Related resource: | https://doi.org/10.1007/978-1-0716-2071-7_9 |
| ISBN: | 978-1-0716-2071-7 |
| Appears in Collections: | Llibres / Capítols de llibre (Bioquímica i Biomedicina Molecular) |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Autophagy in mitotic cells v2.pdf | 613.39 kB | Adobe PDF | View/Open Request a copy |
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