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http://hdl.handle.net/2445/209245
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DC Field | Value | Language |
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dc.contributor.author | Pispa, Johanna | - |
dc.contributor.author | Mikkonen, Elisa | - |
dc.contributor.author | Arpalahti, Leena | - |
dc.contributor.author | Jin, Congyu | - |
dc.contributor.author | Martínez Fernández, Carmen | - |
dc.contributor.author | Cerón, Julián | - |
dc.contributor.author | Holmberg, Carina I. | - |
dc.date.accessioned | 2024-03-26T19:36:36Z | - |
dc.date.available | 2024-03-26T19:36:36Z | - |
dc.date.issued | 2023-10-01 | - |
dc.identifier.issn | 2589-0042 | - |
dc.identifier.uri | http://hdl.handle.net/2445/209245 | - |
dc.description.abstract | Polyubiquitinated proteins are primarily degraded by the ubiquitin-proteasome system (UPS). Proteasomes are present both in the cytoplasm and nucleus. Here, we investigated mechanisms coordinating proteasome subcellular localization and activity in a multicellular organism. We identified the nuclear protein-encoding gene akir-1 as a proteasome regulator in a genome-wide Caenorhabditis elegans RNAi screen. We demonstrate that depletion of akir-1 causes nuclear accumulation of endogenous polyubiquitinated proteins in intestinal cells, concomitant with slower in vivo proteasomal degradation in this subcellular compartment. Remarkably, akir-1 is essential for nuclear localization of proteasomes both in oocytes and intestinal cells but affects differentially the subcellular distribution of polyubiquitinated proteins. We further reveal that importin ima-3 genetically interacts with akir-1 and influences nuclear localization of a polyubiquitin-binding reporter. Our study shows that the conserved AKIR-1 is an important regulator of the subcellular function of proteasomes in a multicellular organism, suggesting a role for AKIR-1 in proteostasis maintenance. | - |
dc.format.extent | 21 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier BV | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1016/j.isci.2023.107886 | - |
dc.relation.ispartof | iScience, 2023, vol. 26, num. 10 | - |
dc.relation.uri | https://doi.org/10.1016/j.isci.2023.107886 | - |
dc.rights | cc by (c) Pispa, Johanna et al., 2023 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.source | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) | - |
dc.subject.classification | Proteïnes | - |
dc.subject.classification | Homeòstasi | - |
dc.subject.other | Proteins | - |
dc.subject.other | Homeostasis | - |
dc.title | AKIR-1 regulates proteasome subcellular function in Caenorhabditis elegans | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.date.updated | 2023-11-22T08:57:16Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 37767001 | - |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
Files in This Item:
File | Description | Size | Format | |
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PIIS2589004223019636.pdf | 4.06 MB | Adobe PDF | View/Open |
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