Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/209822
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DC Field | Value | Language |
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dc.contributor.author | Eills, James | - |
dc.contributor.author | Picazo Frutos, Roman | - |
dc.contributor.author | Bondar, Oksana | - |
dc.contributor.author | Cavallari, Eleonora | - |
dc.contributor.author | Carrera, Carla | - |
dc.contributor.author | Barker, Sylwia J | - |
dc.contributor.author | Utz, Marcel | - |
dc.contributor.author | Herrero Gómez, Alba | - |
dc.contributor.author | Marco Rius, Irene | - |
dc.contributor.author | Tayler, Michael C. D. | - |
dc.contributor.author | Aime, Silvio | - |
dc.contributor.author | Reineri, Francesca | - |
dc.contributor.author | Budker, Dmitry | - |
dc.contributor.author | Blanchard, John W | - |
dc.date.accessioned | 2024-04-12T09:40:38Z | - |
dc.date.available | 2024-04-12T09:40:38Z | - |
dc.date.issued | 2023-12-04 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | http://hdl.handle.net/2445/209822 | - |
dc.description.abstract | We demonstrate that enzyme-catalyzed reactions can be observed in zero- and low-field NMR experiments by combining recent advances in parahydrogen-based hyperpolarization methods with state-of-the-art magnetometry. Specifically, we investigated two model biological processes: the conversion of fumarate into malate, which is used in vivo as a marker of cell necrosis, and the conversion of pyruvate into lactate, which is the most widely studied metabolic process in hyperpolarization-enhanced imaging. In addition to this, we constructed a microfluidic zero-field NMR setup to perform experiments on microliter-scale samples of [1-C-13]-fumarate in a lab-on-a-chip device. Zero- to ultralow-field (ZULF) NMR has two key advantages over high-field NMR: the signals can pass through conductive materials (e.g., metals), and line broadening from sample heterogeneity is negligible. To date, the use of ZULF NMR for process monitoring has been limited to studying hydrogenation reactions. In this work, we demonstrate this emerging analytical technique for more general reaction monitoring and compare zero- vs low-field detection. | - |
dc.format.extent | 9 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1021/acs.analchem.3c02087 | - |
dc.relation.ispartof | Analytical Chemistry, 2023, vol. 95, num. 49 p. 17997-18005 | - |
dc.relation.uri | https://doi.org/10.1021/acs.analchem.3c02087 | - |
dc.rights | (c) Eills, James et al, 2023 | - |
dc.source | Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) | - |
dc.subject.classification | Ressonància magnètica | - |
dc.subject.classification | Hidrogenació | - |
dc.subject.other | Magnetic resonance | - |
dc.subject.other | Hydrogenation | - |
dc.title | Enzymatic Reactions Observed with Zero- and Low-Field Nuclear Magnetic Resonance | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.date.updated | 2024-01-31T12:44:07Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.idimarina | 6605961 | - |
dc.identifier.pmid | 38047582 | - |
Appears in Collections: | Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) |
Files in This Item:
File | Description | Size | Format | |
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2023_AnalChem_Enzymatic_EillsJ.pdf | 4.12 MB | Adobe PDF | View/Open |
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