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Title: | A 3D bioprinted hydrogel gut-on-chip with integrated electrodes for transepithelial electrical resistance (TEER) measurements |
Author: | Vera, Daniel García Díaz, María Torras, Nuria Castillo, Óscar Illa, Xavi Villa, Rosa Álvarez, Mar Martínez, Elena |
Keywords: | Impressió 3D Mucosa gastrointestinal Three-dimensional printing Gastrointestinal mucosa |
Issue Date: | 1-Jul-2024 |
Abstract: | Conventional gut-on-chip (GOC) models typically represent the epithelial layer of the gut tissue, neglecting other important components such as the stromal compartment and the extracellular matrix (ECM) that play crucial roles in maintaining intestinal barrier integrity and function. These models often employ hard, flat porous membranes for cell culture, thus failing to recapitulate the soft environment and complex 3D architecture of the intestinal mucosa. Alternatively, hydrogels have been recently introduced in GOCs as ECM analogs to support the co-culture of intestinal cells in in vivo-like configurations, and thus opening new opportunities in the organ-on-chip field. In this work, we present an innovative GOC device that includes a 3D bioprinted hydrogel channel replicating the intestinal villi architecture containing both the epithelial and stromal compartments of the gut mucosa. The bioprinted hydrogels successfully support both the encapsulation of fibroblasts and their co-culture with intestinal epithelial cells under physiological flow conditions. Moreover, we successfully integrated electrodes into the microfluidic system to monitor the barrier formation in real time via transepithelial electrical resistance measurements. |
Note: | Reproducció del document publicat a: https://doi.org/10.1088/1758-5090/ad3aa4 |
It is part of: | Biofabrication, 2024, vol. 16, num. 3 |
URI: | http://hdl.handle.net/2445/211240 |
Related resource: | https://doi.org/10.1088/1758-5090/ad3aa4 |
ISSN: | 1758-5090 |
Appears in Collections: | Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC)) |
Files in This Item:
File | Description | Size | Format | |
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2024_Biofabrication_A3D_MartinezE.pdf | 2.52 MB | Adobe PDF | View/Open |
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