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http://hdl.handle.net/2445/213560
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DC Field | Value | Language |
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dc.contributor.author | PERDICES SEGUI, Quim | - |
dc.contributor.author | El-Khoury R | - |
dc.contributor.author | Cabrero C | - |
dc.contributor.author | MOVILLA NUÑEZ, Santiago | - |
dc.contributor.author | Kaur H | - |
dc.contributor.author | Friedland D | - |
dc.contributor.author | Domínguez À | - |
dc.contributor.author | Thorpe JD | - |
dc.contributor.author | Roman M | - |
dc.contributor.author | Orozco Lopez, Modesto | - |
dc.contributor.author | Gonzalez C | - |
dc.contributor.author | Damha MJ | - |
dc.date.accessioned | 2024-06-25T16:53:38Z | - |
dc.date.available | 2024-06-25T16:53:38Z | - |
dc.date.issued | 2024-06-14 | - |
dc.identifier.issn | El-Khoury R; Cabrero C; Movilla S; Kaur H; Friedland D; Domínguez A; Thorpe JD; Roman M; Orozco M; González C; Damha MJ (2024). Formation of left-handed helices by C2'-fluorinated nucleic acids under physiological salt conditions.. Nucleic Acids Research, (), -. DOI: 10.1093/nar/gkae508 | - |
dc.identifier.uri | http://hdl.handle.net/2445/213560 | - |
dc.description.abstract | Recent findings in cell biology have rekindled interest in Z-DNA, the left-handed helical form of DNA. We report here that two minimally modified nucleosides, 2'F-araC and 2'F-riboG, induce the formation of the Z-form under low ionic strength. We show that oligomers entirely made of these two nucleosides exclusively produce left-handed duplexes that bind to the Zα domain of ADAR1. The effect of the two nucleotides is so dramatic that Z-form duplexes are the only species observed in 10 mM sodium phosphate buffer and neutral pH, and no B-form is observed at any temperature. Hence, in contrast to other studies reporting formation of Z/B-form equilibria by a preference for purine glycosidic angles in syn, our NMR and computational work revealed that sequential 2'F…H2N and intramolecular 3'H…N3' interactions stabilize the left-handed helix. The equilibrium between B- and Z- forms is slow in the 19F NMR time scale (≥ms), and each conformation exhibited unprecedented chemical shift differences in the 19F signals. This observation led to a reliable estimation of the relative population of B and Z species and enabled us to monitor B-Z transitions under different conditions. The unique features of 2'F-modified DNA should thus be a valuable addition to existing techniques for specific detection of new Z-binding proteins and ligands. | - |
dc.format.extent | null | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | English | - |
dc.relation.isformatof | https://doi.org/10.1093/nar/gkae508 | - |
dc.relation.ispartof | Nucleic Acids Research, 2024, | - |
dc.relation.uri | https://doi.org/10.1093/nar/gkae508 | - |
dc.source | Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona)) | - |
dc.subject | Astronomia / física | - |
dc.subject | Biochemistry & molecular biology | - |
dc.subject | Biodiversidade | - |
dc.subject | Biotecnología | - |
dc.subject | Ciência da computação | - |
dc.subject | Ciências agrárias i | - |
dc.subject | Ciências biológicas i | - |
dc.subject | Ciências biológicas ii | - |
dc.subject | Ciências biológicas iii | - |
dc.subject | Engenharias ii | - |
dc.subject | Farmacia | - |
dc.subject | Genetics | - |
dc.subject | Interdisciplinar | - |
dc.subject | Matemática / probabilidade e estatística | - |
dc.subject | Medicina i | - |
dc.subject | Medicina ii | - |
dc.subject | Nutrição | - |
dc.subject | Química | - |
dc.subject | Saúde coletiva | - |
dc.subject | Zootecnia / recursos pesqueiros | - |
dc.title | Formation of left-handed helices by C2′-fluorinated nucleic acids under physiological salt conditions | - |
dc.type | article | - |
dc.date.updated | 2024-06-21T07:08:08Z | - |
dc.identifier.idimarina | 6618425 | - |
Appears in Collections: | Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona)) |
Files in This Item:
File | Description | Size | Format | |
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SMovilla_MOrozco_NAR_full_2024.pdf | 4.54 MB | Adobe PDF | View/Open |
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