Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/216697
Full metadata record
DC FieldValueLanguage
dc.contributor.authorDíaz-Sáez, Francisco-
dc.contributor.authorBalcells Nadal, Cristina-
dc.contributor.authorRosselló, Laura-
dc.contributor.authorLópez-Soldado, Iliana-
dc.contributor.authorRomero, Montserrat-
dc.contributor.authorSebastián Muñoz, David-
dc.contributor.authorLópez-Soriano, Francisco J.-
dc.contributor.authorBusquets Rius, Sílvia-
dc.contributor.authorCascante i Serratosa, Marta-
dc.contributor.authorRicart, Wifredo-
dc.contributor.authorFernández-Real Lemos, José Manuel-
dc.contributor.authorMoreno-Navarrete, José María-
dc.contributor.authorAragonés, Julián-
dc.contributor.authorTestar, Xavier-
dc.contributor.authorCamps Camprubí, Marta-
dc.contributor.authorZorzano Olarte, Antonio-
dc.contributor.authorGumà i Garcia, Anna Maria-
dc.date.accessioned2024-11-22T15:43:44Z-
dc.date.available2024-11-22T15:43:44Z-
dc.date.issued2024-11-01-
dc.identifier.issn1661-6596-
dc.identifier.urihttps://hdl.handle.net/2445/216697-
dc.description.abstractNeuregulin 4 (Nrg4) is an adipokine that belongs to the epidermal growth factor family andbinds to ErbB4 tyrosine kinase receptors. In 3T3-L1 adipocytes, the downregulation of Nrg4 expressionenhances inflammation and autophagy, resulting in insulin resistance. Here, we searched for thecauses of this phenotype. Nrg4 knockdown (Nrg4 KD) adipocytes showed a significant reduction inmitochondrial content and elongation, along with a lower content of the mitochondria fusion proteinmitofusin 2 (MFN2), and increased H2O2 production compared to the control scrambled cells (Scr).The antioxidant N-acetylcysteine reversed the oxidative stress and reduced the gene expression of thepro-inflammatory cytokine tumor necrosis factor α (TNFα). Nrg4 KD adipocytes showed enhancedlipolysis and reduced lipogenesis, in addition to a significant reduction in several intermediatesof the Krebs cycle. In summary, Nrg4 downregulation in adipocytes affects mitochondrial contentand functioning, causing impaired cellular metabolism, which in turn results in oxidative stress,inflammation, and insulin resistance.-
dc.format.extent18 p.-
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherMDPI-
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.3390/ijms252111718-
dc.relation.ispartofInternational Journal of Molecular Sciences, 2024, vol. 25, num.21, p. 1-18-
dc.relation.urihttps://doi.org/10.3390/ijms252111718-
dc.rightscc-by (c) Francisco Díaz-Sáez et al., 2024-
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/-
dc.sourceArticles publicats en revistes (Bioquímica i Biomedicina Molecular)-
dc.subject.classificationMitocondris-
dc.subject.classificationEstrès oxidatiu-
dc.subject.classificationInflamació-
dc.subject.classificationResistència a la insulina-
dc.subject.otherMitochondria-
dc.subject.otherOxidative stress-
dc.subject.otherInflammation-
dc.subject.otherInsulin resistance-
dc.titleNeuregulin 4 downregulation alters mitochondrial morphology and induces oxidative stress in 3T3-L1 adipocytes-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.identifier.idgrec751287-
dc.date.updated2024-11-22T15:43:45Z-
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess-
Appears in Collections:Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
Articles publicats en revistes (Institut de Biomedicina (IBUB))
Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))

Files in This Item:
File Description SizeFormat 
869200.pdf2.02 MBAdobe PDFView/Open


This item is licensed under a Creative Commons License Creative Commons