Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/217716
Title: Endometrial epithelial cell organoids as tools for studying the CD39 family of enzymes and for validating enzyme inhibitors
Author: Rodríguez-Martínez, Aitor
Torrejón-Escribano, Benjamín
Eritja, Núria
Dorca Arévalo, Jonatan
Gabaldón, Clara
Sévigny, Jean
Matias-Guiu, Xavier, 1958-
Martín Satué, Mireia
Keywords: Càncer d'endometri
Cèl·lules epitelials
Citoquímica
Endometrial cancer
Epithelial cells
Cytochemistry
Issue Date: 17-Jun-2024
Publisher: Sercrisma International
Abstract: Extracellular adenosine triphosphate (ATP) conducts a complex dynamic system of broadly represented cell signaling. Ectonucleotidases are the enzymes with nucleotide hydrolytic ability that regulate ATP levels in physiological and pathological conditions, thus playing a key role in the so-called purinergic signaling. Altered ectonucleotidase expression has been reported in cancer, and the ectonucleoside triphosphate diphosphohydrolase (NTPDase) family of enzymes, with its best-known form NTPDase1 (CD39), is targeted in cancer immunotherapy. The tandem of enzymes CD39-CD73 is responsible for the generation of immuno-suppressive adenosine in the tumor microenvironment, and inhibition strategies are of great interest. Organoids have emerged as very convenient models for the study of tumors since they are three-dimensional cultures that retain many of the features of tissue. The present study aims to contribute to improving the methodology and the molecular tools needed for the study of ecto-nucleotidases in healthy and disease conditions. The study, performed in an endometrial cancer cell model, could be extended to other types of tumors and pathologies in which the purinergic system is involved. We generated organoids from endometrial cancer cells overexpressing NTPDase2 (CD39L1) and NTPDase3 (CD39L3) as fusion proteins with EGFP, and we performed functional assays by adapting in situ cytochemistry protocols. This allowed us to simultaneously detect enzyme activity and protein expression and to demonstrate that organoids can be used to test ectonucleotidase inhibitors—a result that can be used to develop new cancer treatment options.
Note: Reproducció del document publicat a: https://doi.org/10.14670/HH-18-782
It is part of: Histology and Histopathology, 2024, vol. 39, p. 171-182
URI: https://hdl.handle.net/2445/217716
Related resource: https://doi.org/10.14670/HH-18-782
ISSN: 0213-3911
Appears in Collections:Articles publicats en revistes (Patologia i Terapèutica Experimental)

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