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https://hdl.handle.net/2445/218881
Title: | Using anal cytology and HPV DNA and E6/E7 mRNA detection to optimize high-resolution anoscopy referrals in men who have sex with men living with HIV |
Author: | Silva klug, Ana C Paytubi Casabona, Sònia Torres, Montserrat Trenti, Loris Baixeras, Nuria Sanchez Llamas, Monica Pavón, Miquel Ángel Sanjose, Silvia De Catala, Isabel Vidal, August Poljak, Mario Alemany, Laia Podzamczer Palter, Daniel Videla, Sebastian Saumoy, Maria |
Keywords: | Malalties de l'anus Papil·lomavirus VIH (Virus) Citologia Diseases of the anus Papillomaviruses HIV (Viruses) Cytology |
Issue Date: | 23-Dec-2024 |
Publisher: | Oxford University Press (OUP) |
Abstract: | Background This study was conducted to evaluate screening procedures for anal high-grade squamous intraepithelial lesions (HSILs) with anal liquid-based cytology (aLBC) and biomarkers to identify candidates for high-resolution anoscopy (HRA).Methods This cross-sectional study included men who have sex with men with HIV. Participants underwent HRA, aLBC, and biomarker testing. Three screening procedures were compared with aLBC: biomarker alone, cytology and biomarker in all, and cytology and reflex biomarkers (biomarkers applied if aLBC results were atypical squamous cells of undetermined significance or low-grade squamous intraepithelial lesion). Biomarkers included Linear Array (LA), LA for 14 high-risk human papillomavirus (LA 14 HR-HPV) genotypes, LA HPV-16, Hybrid Capture 2 (HC2), E6/E7 mRNA, and E6/E7 mRNA HPV-16.Results Of 354 participants, 179 (50.6%) had atypical squamous cells of undetermined significance or worse, requiring HRA (sensitivity, 80%; specificity, 57.3%; area under the curve, 0.687; reference, biopsy-proven HSIL). Cytology and reflex biomarkers per E6/E7 mRNA, LA 14 HR-HPV, and HC2 and the biomarker-alone procedure with HC2 showed comparable accuracy (sensitivities: 71.6%, 78.8%, 73.1%, 75.7%; specificities: 73.5%, 67.9%, 76.1%, 65.5%; areas under the curve: 0.726, 0.734, 0.746, 0.706) with fewer HRA referrals (number needed to diagnose: 2.2, 2.1, 2, 2.4).Conclusions Our findings suggest that E6/E7 mRNA, LA 14 HR-HPV, and HC2 in the cytology and reflex biomarkers procedure, as well as HC2 in the biomarker-alone procedure, can improve anal HSIL screening effectiveness. |
Note: | Reproducció del document publicat a: https://doi.org/10.1093/ofid/ofae735 |
It is part of: | Open Forum Infectious Diseases, 2024, vol. 12, num. 1 |
URI: | https://hdl.handle.net/2445/218881 |
Related resource: | https://doi.org/10.1093/ofid/ofae735 |
ISSN: | 2328-8957 |
Appears in Collections: | Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) Articles publicats en revistes (ISGlobal) |
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