Please use this identifier to cite or link to this item:
https://hdl.handle.net/2445/220777
Title: | Visualizing G protein-coupled receptor-receptor interactions in brain using proximity ligation in situ assay |
Author: | Taura, Jaume López-Cano, Marc Fernández Dueñas, Víctor Ciruela Alférez, Francisco |
Keywords: | Cervell Proteïnes portadores Fixació de proteïnes Brain Carrier proteins Protein binding |
Issue Date: | 7-Jun-2023 |
Abstract: | G protein-coupled receptors (GPCRs) constitute the largest family of plasma membrane receptors and the main drug targets in therapeutics. GPCRs can establish direct receptor-receptor interactions (oligomerization), which can also be considered as targets for drug development (GPCR oligomer-based drugs). However, prior to designing any novel GPCR oligomer-based drug development program, demonstrating the existence of a named GPCR oligomer in native tissues is needed as part of its target engagement definition. Here, we discuss the proximity ligation in situ assay (P-LISA), an experimental approach that reveals GPCR oligomerization in native tissues. We provide a detailed step-by-step protocol to perform P-LISA experiments and visualize GPCR oligomers in brain slices. We also provide instructions for slide observation, data acquisition, and quantification. Finally, we discuss the critical aspects determining the success of the technique, namely the fixation process and the validation of the primary antibodies used. Overall, this protocol may be used to straightforwardly visualize GPCR oligomers in the brain. |
Note: | Reproducció del document publicat a: https://doi.org/10.1002/cpz1.794 |
It is part of: | 2023, vol. 3, num.6 |
URI: | https://hdl.handle.net/2445/220777 |
Related resource: | https://doi.org/10.1002/cpz1.794 |
ISSN: | 1934-2500 |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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