Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/221882
Title: Basal association of a transcription factor favors early gene expression
Author: Pinheiro, Sandrine
Nadal Ribelles, Mariona
Solé Serra, Carme
Vincenzetti, Vincent
Dusserre, Yves
Posas, Francesc
Pelet, Serge
Keywords: Factors de transcripció
Regulació genètica
Regulació cel·lular
Transcription factors
Genetic regulation
Cellular control mechanisms
Issue Date: 16-Jun-2025
Publisher: Plos
Abstract: Responses to extracellular signals via Mitogen-Activated Protein Kinase (MAPK) pathways control complex transcriptional programs where hundreds of genes are induced at a desired level with a specific timing. Gene expression regulation is largely encoded in the promoter of the gene, which harbors numerous transcription factor binding sites. In the mating MAPK pathway of Saccharomyces cerevisiae, one major transcription factor, Ste12, controls the chronology of gene expression necessary for the fusion of two haploid cells. Because endogenous promoters encode a large diversity of Ste12 binding sites (PRE), we engineered synthetic promoters to decipher the rules that dictate mating gene induction. Conformations of PRE dimers that allow efficient gene expression were identified. The strength of binding of Ste12 to the PRE and the distance of the binding sites to the core promoter modulate the level of induction. The speed of activation is ensured by favoring a basal association of Ste12 by using a strong dimer of PRE located in a nucleosome depleted region.
Note: Reproducció del document publicat a: https://doi.org/10.1371/journal.pgen.1011710
It is part of: PLoS Genetics, 2025, vol. 21, num. 6
URI: https://hdl.handle.net/2445/221882
Related resource: https://doi.org/10.1371/journal.pgen.1011710
ISSN: 1553-7404
Appears in Collections:Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))

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