Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/221890
Title: Ketorolac, melatonin and latanoprost tri-loaded PLGA microspheres for neuroprotection in glaucoma
Author: González-Cela Casamayor, Miriam Ana
Rodrigo Sanjuán, Maria Jesús
Brugnera, Marco
Munuera, Inés
Martínez Rincón, Teresa
Prats Lluís, Catalina
Villacampa, Pilar
García Feijoo, Julian
Pablo-Júlvez, Luis E.
Bravo Osuna, Irene
García Martñin, Elena
Herrero Vanrell, Rocío
Keywords: Melatonina
Glaucoma
Terapèutica oftalmològica
Melatonin
Glaucoma
Ophthalmological therapeutics
Issue Date: 11-Apr-2025
Publisher: Informa UK Limited
Abstract: Glaucoma is a multifactorial neurodegenerative disease that affects the retina and optic nerve. The aim of this work was to reach different therapeutics targets by co-encapsulating three neuroprotective substances with hypotensive (latanoprost), antioxidant (melatonin) and anti-inflammatory (ketorolac) activity in biodegradable poly (lactic-co-glycolic acid) (PLGA) microspheres (MSs) capable of releasing the drugs for months after intravitreal injection, avoiding the need for repeated administrations. Multi-loaded PLGA MSs were prepared using the oil-in-water emulsion solvent extraction-evaporation technique and physicochemically characterized. PLGA 85:15 was the polymer ratio selected for the selected formulation. Tri-loaded MSs including vitamin E as additive showed good tolerance in retinal pigment epithelium cells after 24 h exposure (>90% cell viability). The final formulation (KMLVE) resulted in 33.58 +/- 5.44 mu m particle size and drug content (mu g/mg MSs) of 39.70 +/- 5.89, 67.28 +/- 4.17 and 7.51 +/- 0.58 for melatonin, ketorolac and latanoprost respectively. KMLVE were able to release in a sustained manner the three drugs over 70 days. KMLVE were injected at 2 and 12 weeks in Long-Evans rats (n = 20) after the induction of chronic glaucoma. Ophthalmological tests were performed and compared to not treated glaucomatous (n = 45) and healthy (n = 17) animals. Treated glaucomatous rats reached the lowest intraocular pressure, enhanced functionality of bipolar and retinal ganglion cells and showed greater neuroretinal thickness by optical coherence tomography (p < 0.05) compared to not treated glaucomatous rats at 24 weeks follow-up. According to the results, the tri-loaded microspheres can be considered as promising controlled-release system for the treatment of glaucoma.
Note: Reproducció del document publicat a: https://doi.org/10.1080/10717544.2025.2484277
It is part of: Drug Delivery, 2025, vol. 32, num. 1
URI: https://hdl.handle.net/2445/221890
Related resource: https://doi.org/10.1080/10717544.2025.2484277
ISSN: 1521-0464
Appears in Collections:Articles publicats en revistes (Ciències Fisiològiques)
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))



This item is licensed under a Creative Commons License Creative Commons