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https://hdl.handle.net/2445/222060
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DC Field | Value | Language |
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dc.contributor.author | Calderón Gómez, Elisabeth | - |
dc.contributor.author | Bassolas Molina, Helena | - |
dc.contributor.author | Mora Buch, Rut | - |
dc.contributor.author | Dotti, Isabella | - |
dc.contributor.author | Planell Picola, Núria | - |
dc.contributor.author | Esteller Viñal, Miriam | - |
dc.contributor.author | Gallego Barrero, Marta | - |
dc.contributor.author | Martí Ripoll, Maria Mercè | - |
dc.contributor.author | Garcia Martin, Carme | - |
dc.contributor.author | Martínez Torro, Carlos | - |
dc.contributor.author | Ordas, Ingrid | - |
dc.contributor.author | Singh, Sharat | - |
dc.contributor.author | Panes, Julian | - |
dc.contributor.author | Benítez Ribas, Daniel | - |
dc.contributor.author | Salas, Azucena | - |
dc.date.accessioned | 2025-07-07T16:50:22Z | - |
dc.date.available | 2025-07-07T16:50:22Z | - |
dc.date.issued | 2016-09-01 | - |
dc.identifier.issn | 0016-5085 | - |
dc.identifier.uri | https://hdl.handle.net/2445/222060 | - |
dc.description.abstract | BACKGROUND & AIMS: Crohn's disease (CD) has been associated with an altered immune response to commensal microbiota, mostly based on increased seroreactivity to microbial proteins. Although T cells are believed to contribute to the development of CD, little is known about the antigens involved. We investigated the antigen-specificity of T cells isolated from patients with CD. METHODS: We isolated peripheral blood mononuclear cells from 65 patients with CD and 45 healthy individuals (controls). We investigated T-cell reactivity to commensal microbial antigens using proliferation assays (based on thymidine incorporation and carboxyfluorescein succinimidyl ester dilution). Gene expression patterns were determined using microarray and real-time polymerase chain reaction analyses. Cytokines, chemokines, and antibodies were measured by enzyme-linked immunosorbent assay, flow cytometry, or multiplex cytokine assays. Intestinal crypts were obtained from surgical resection specimens of 7 individuals without inflammatory bowel disease. We examined the effects of commensal-specific CD4(+) T cells on primary intestinal epithelial cells from these samples. RESULTS: The bacterial proteins FlaX, A4-fla2, and YidX increased proliferation of CD4(+) T cells isolated from peripheral blood of patients with CD compared with controls. In blood samples from controls, CD4(+) T cells specific for FlaX, A4-fla2, or YidX had a T-helper (Th) 1 phenotype; a larger proportion of CD4(+) T cells specific for these proteins in patients with CD had a Th17 phenotype or produced Th1 and Th17 cytokines. When supernatants collected from commensal-specific CD4(+) T cells from patients with CD were applied to healthy intestinal epithelial cells, the epithelial cells increased the expression of the chemokine (C-X-C motif) ligand 1 (CXCL1), CXCL8 and the CC chemokine ligand 20 (CCL20). CONCLUSIONS: A larger proportion of commensal-specific CD4(+) T cells from patients with CD have a Th17 phenotype or produce Th1 and Th17 cytokines, compared with T cells from controls; this might contribute to intestinal inflammation in patients with CD. These cells might be targeted for treatment of CD. The transcriptional data of commensal-specific CD4(+) T cells from healthy individuals and CD patients have been deposited in the Gene Expression Omnibus at the National Center for Biotechnology Information (accession no: GSE70469). | - |
dc.format.extent | 15 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier | - |
dc.relation.isformatof | Reproducció del document publicat a: https://doi.org/10.1053/j.gastro.2016.05.050 | - |
dc.relation.ispartof | Gastroenterology, 2016, vol. 151, num.3, p. 489 | - |
dc.relation.uri | https://doi.org/10.1053/j.gastro.2016.05.050 | - |
dc.rights | cc-by-nc-nd (c) AGA Institute, 2016 | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
dc.source | Articles publicats en revistes (Ciències Clíniques) | - |
dc.title | Commensal-Specific CD4(+) Cells From Patients With Crohn's Disease Have a T-Helper 17 Inflammatory Profile | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 676870 | - |
dc.date.updated | 2025-07-07T16:50:22Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 27267052 | - |
Appears in Collections: | Articles publicats en revistes (Ciències Clíniques) Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer) |
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206323.pdf | 2.21 MB | Adobe PDF | View/Open |
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