Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/222060
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dc.contributor.authorCalderón Gómez, Elisabeth-
dc.contributor.authorBassolas Molina, Helena-
dc.contributor.authorMora Buch, Rut-
dc.contributor.authorDotti, Isabella-
dc.contributor.authorPlanell Picola, Núria-
dc.contributor.authorEsteller Viñal, Miriam-
dc.contributor.authorGallego Barrero, Marta-
dc.contributor.authorMartí Ripoll, Maria Mercè-
dc.contributor.authorGarcia Martin, Carme-
dc.contributor.authorMartínez Torro, Carlos-
dc.contributor.authorOrdas, Ingrid-
dc.contributor.authorSingh, Sharat-
dc.contributor.authorPanes, Julian-
dc.contributor.authorBenítez Ribas, Daniel-
dc.contributor.authorSalas, Azucena-
dc.date.accessioned2025-07-07T16:50:22Z-
dc.date.available2025-07-07T16:50:22Z-
dc.date.issued2016-09-01-
dc.identifier.issn0016-5085-
dc.identifier.urihttps://hdl.handle.net/2445/222060-
dc.description.abstractBACKGROUND & AIMS: Crohn's disease (CD) has been associated with an altered immune response to commensal microbiota, mostly based on increased seroreactivity to microbial proteins. Although T cells are believed to contribute to the development of CD, little is known about the antigens involved. We investigated the antigen-specificity of T cells isolated from patients with CD. METHODS: We isolated peripheral blood mononuclear cells from 65 patients with CD and 45 healthy individuals (controls). We investigated T-cell reactivity to commensal microbial antigens using proliferation assays (based on thymidine incorporation and carboxyfluorescein succinimidyl ester dilution). Gene expression patterns were determined using microarray and real-time polymerase chain reaction analyses. Cytokines, chemokines, and antibodies were measured by enzyme-linked immunosorbent assay, flow cytometry, or multiplex cytokine assays. Intestinal crypts were obtained from surgical resection specimens of 7 individuals without inflammatory bowel disease. We examined the effects of commensal-specific CD4(+) T cells on primary intestinal epithelial cells from these samples. RESULTS: The bacterial proteins FlaX, A4-fla2, and YidX increased proliferation of CD4(+) T cells isolated from peripheral blood of patients with CD compared with controls. In blood samples from controls, CD4(+) T cells specific for FlaX, A4-fla2, or YidX had a T-helper (Th) 1 phenotype; a larger proportion of CD4(+) T cells specific for these proteins in patients with CD had a Th17 phenotype or produced Th1 and Th17 cytokines. When supernatants collected from commensal-specific CD4(+) T cells from patients with CD were applied to healthy intestinal epithelial cells, the epithelial cells increased the expression of the chemokine (C-X-C motif) ligand 1 (CXCL1), CXCL8 and the CC chemokine ligand 20 (CCL20). CONCLUSIONS: A larger proportion of commensal-specific CD4(+) T cells from patients with CD have a Th17 phenotype or produce Th1 and Th17 cytokines, compared with T cells from controls; this might contribute to intestinal inflammation in patients with CD. These cells might be targeted for treatment of CD. The transcriptional data of commensal-specific CD4(+) T cells from healthy individuals and CD patients have been deposited in the Gene Expression Omnibus at the National Center for Biotechnology Information (accession no: GSE70469).-
dc.format.extent15 p.-
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherElsevier-
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.1053/j.gastro.2016.05.050-
dc.relation.ispartofGastroenterology, 2016, vol. 151, num.3, p. 489-
dc.relation.urihttps://doi.org/10.1053/j.gastro.2016.05.050-
dc.rightscc-by-nc-nd (c) AGA Institute, 2016-
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.sourceArticles publicats en revistes (Ciències Clíniques)-
dc.titleCommensal-Specific CD4(+) Cells From Patients With Crohn's Disease Have a T-Helper 17 Inflammatory Profile-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/publishedVersion-
dc.identifier.idgrec676870-
dc.date.updated2025-07-07T16:50:22Z-
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess-
dc.identifier.pmid27267052-
Appears in Collections:Articles publicats en revistes (Ciències Clíniques)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)

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