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Title: | New models for MPNST: establishment and comprehensive characterization of two tumor cell lines |
Author: | Ortega Bertran, Sara Creus Bachiller, Edgar Magallón Lorenz, Miriam Carrió, Meritxell Gel Moreno, Bernat Villanueva Garatachea, Alberto Lopez-Gutierrez, Juan Carlos Estival, Anna Serra, Eduard Fernández Rodríguez, Juana Lázaro García, Conxi |
Keywords: | Tumors Regulació cel·lular Neurofibromatosi Tumors Cellular control mechanisms Neurofibromatosis |
Issue Date: | 18-Jul-2025 |
Publisher: | BioMed Central |
Abstract: | Background: Malignant peripheral nerve sheath tumors (MPNSTs) are rare, invasive, and aggressive soft tissue sarcomas arising from peripheral nerves. They may occur sporadically or in association with Neurofibromatosis type 1 (NF1), in which they are the leading cause of mortality. Currently, there are no effective therapies other than surgery. Therefore, tumor-derived cell lines are essential for testing new therapeutic strategies, especially when used in parallel with in vivo models. In this study, we present two new MPNST cell lines and two patient-derived orthotopic xenograft (PDOX) models from a sporadic (SP-10) and an NF1-related (NF1-18B) MPNST patient to increase the number of available preclinical models for in vitro and in vivo drug testing. Methods: The cell lines were isolated and extensively characterized genetically (tumor suppressor gene mutation status, DNA content), phenotypically (cell morphology, marker expression), and functionally (proliferation rate, colony formation capacity, migration rate, tumorigenic ability). We validated the models by comparing the genomic (copy number variation profile) and histological characteristics of the cell lines and PDOX tumors with their corresponding patient tumors. Results: The new cell lines and PDOXs tumors exhibited similar genomic copy number variation profiles, histological patterns, and marker expressions as the patient tumors, validating them as faithful models. Interestingly, the NF1-18B cell model presented two cell subpopulations with different ploidy states (one < 3n and the other 4n) and functional features in vitro. NF1-18B 4n, along with SP-10 cell lines, exhibited in vitro functional hallmarks of MPNSTs, including high proliferation and migration rates and colony forming ability. However, only the SP-10 model exhibited aggressive tumorigenicity in athymic mice. In contrast, the NF1-18B < 3n showed a low migration rate and did not form colonies or aggregates in vitro. Conclusions: The newly established MPNST cell lines, along with their corresponding PDOX models, serve as valuable tools for both in vitro and in vivo testing of novel therapeutic agents. Notably, the SP-10 cell line model represents one of the few documented cases isolated from a genuine "classic" MPNST. |
Note: | Reproducció del document publicat a: https://doi.org/10.1186/s12935-025-03845-4 |
It is part of: | Cancer Cell International, 2025, vol. 25, num.1 |
URI: | https://hdl.handle.net/2445/222881 |
Related resource: | https://doi.org/10.1186/s12935-025-03845-4 |
ISSN: | 1475-2867 |
Appears in Collections: | Articles publicats en revistes (Fonaments Clínics) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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