Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/223002
Title: The effect of the shutter exposure time in force calibration in Magnetic Tweezers
Author: Gorgori Diez del Corral, Judit
Director/Tutor: Mañosas Castejón, María
Keywords: Pinces magnètiques
Calibratge
Treballs de fi de grau
Magnetic tweezers
Calibration
Bachelor's theses
Issue Date: Jan-2025
Abstract: Magnetic tweezers is a single-molecule technique highly used in biophysics to investigate processes at molecular scale. Obtaining quantitative results relies on a precise force calibration, which depends on the measurement of bead’s transversal fluctuations. The later is influenced by the camera shutter exposure time along which the light intensity signal is averaged. Previous works have highlight the importance of reducing the camera’s exposure time in short molecules (where the bead’s fluctuations are more rapid). Here we carry out experiments with long and short DNA molecules to study the effect of using different shutter exposure times in force calibrations. We find that working at the standard conditions, where the camera exposure time is maximized, the force is highly overestimated. Whereas modulating the exposure time becomes a good strategy to correctly measure forces both in long and short molecules. Finally, we use the results to compare the elastic response of single-stranded and double-stranded DNA molecules
Note: Treballs Finals de Grau de Física, Facultat de Física, Universitat de Barcelona, Curs: 2025, Tutora: Maria Mañosas
URI: https://hdl.handle.net/2445/223002
Appears in Collections:Treballs Finals de Grau (TFG) - Física

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