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dc.contributor.authorLuna Moñux, Anacat
dc.contributor.authorMatas Guadix, Olga Beléncat
dc.contributor.authorMartínez-Menárguez, José Ángelcat
dc.contributor.authorMato Matute, Maria Eugèniacat
dc.contributor.authorDurán Serrano, Juan Manuelcat
dc.contributor.authorBallesta, Josécat
dc.contributor.authorWay, Michaelcat
dc.contributor.authorEgea Guri, Gustavocat
dc.description.abstractActin is involved in the organization of the Golgi complex and Golgi-to-ER protein transport in mammalian cells. Little, however, is known about the regulation of the Golgi-associated actin cytoskeleton. We provide evidence that Cdc42, a small GTPase that regulates actin dynamics, controls Golgi-to-ER protein transport. We located GFP-Cdc42 in the lateral portions of Golgi cisternae and in COPI-coated and noncoated Golgi-associated transport intermediates. Overexpression of Cdc42 and its activated form Cdc42V12 inhibited the retrograde transport of Shiga toxin from the Golgi complex to the ER, the redistribution of the KDEL receptor, and the ER accumulation of Golgi-resident proteins induced by the active GTP-bound mutant of Sar1 (Sar1[H79G]). Coexpression of wild-type or activated Cdc42 and N-WASP also inhibited Golgito-ER transport, but this was not the case in cells expressing Cdc42V12 and N-WASP(AWA), a mutant form of N-WASP that lacks Arp2/3 binding. Furthermore, Cdc42V12 recruited GFP-NWASP to the Golgi complex. We therefore conclude that Cdc42 regulates Golgi-to-ER protein transport in an N-WASP¿dependent manner.eng
dc.format.extent14 p.-
dc.publisherAmerican Society for Cell Biologyca
dc.relation.isformatofReproducció del document publicat a:
dc.relation.ispartofMolecular Biology of the Cell, 2002, vol. 13, núm. 3, p. 866-879-
dc.rightscc-by-nc-sa (c) Luna et al., 2002-
dc.sourceArticles publicats en revistes (Biomedicina)-
dc.subject.classificationAparell de Golgicat
dc.subject.classificationReticle endoplasmàticcat
dc.subject.classificationTransport biològiccat
dc.subject.otherGolgi apparatuseng
dc.subject.otherEndoplasmic reticulumeng
dc.subject.otherBiological transporteng
dc.titleRegulation of protein transport from the Golgi complex to the endoplasmic reticulum by CDC42 and N-WASP.eng
Appears in Collections:Articles publicats en revistes (Biomedicina)

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