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Title: Diacylglycerol is required for the formation of COPI vesicles in the Golgi-to-ER transport pathway
Author: Sarri Plans, Elisabet
Martínez Rodríguez, Susana Eva
Jiménez, Nuria
Claro Izaguirre, Enrique
Mérida, Isabel
Burger, Koert N. J.
Egea Guri, Gustavo
Fernández-Ulibarri, Inés
Vilella, Montserrat
Lázaro Diégez, Francisco
Keywords: Aparell de Golgi
Reticle endoplasmàtic
Transport biològic
Golgi apparatus
Endoplasmic reticulum
Biological transport
Issue Date: 2007
Publisher: American Society for Cell Biology
Abstract: Diacylglycerol is necessary for trans-Golgi network (TGN) to cell surface transport, but its functional relevance in the early secretory pathway is unclear. Although depletion of diacylglycerol did not affect ER-to-Golgi transport, it led to a redistribution of the KDEL receptor to the Golgi, indicating that Golgi-to-ER transport was perturbed. Electron microscopy revealed an accumulation of COPI-coated membrane profiles close to the Golgi cisternae. Electron tomography showed that the majority of these membrane profiles originate from coated buds, indicating a block in membrane fission. Under these conditions the Golgi-associated pool of ARFGAP1 was reduced, but there was no effect on the binding of coatomer or the membrane fission protein CtBP3/BARS to the Golgi. The addition of 1,2-dioctanoyl-sn-glycerol or the diacylglycerol analogue phorbol 12,13-dibutyrate reversed the effects of endogenous diacylglycerol depletion. Our findings implicate diacylglycerol in the retrograde transport of proteins from Golgi to the ER and suggest that it plays a critical role at a late stage of COPI vesicle formation.
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It is part of: Molecular Biology of the Cell, 2007, vol. 18, núm. 9, p. 3250-3263
ISSN: 1059-1524
Appears in Collections:Articles publicats en revistes (Biomedicina)

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