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Title: ß-Catenin Regulation during the Cell Cycle: Implications in G2/M and Apoptosis
Author: Olmeda, David
Castel i Gil, Susanna
Vilaró, Senén, 1956-2005
Cano, Amparo
Keywords: Apoptosi
Cicle cel·lular
Proteïnes citosquelètiques
Cell cycle
Cytoskeletal proteins
Issue Date: 11-Mar-2003
Publisher: American Society for Cell Biology
Abstract: ß-catenin is a multifunctional protein involved in cell-cell adhesion and Wnt signal transduction. ß-Catenin signaling has been proposed to act as inducer of cell proliferation in different tumors. However, in some developmental contexts and cell systems ß-catenin also acts as a positive modulator of apoptosis. To get additional insights into the role of ß-Catenin in the regulation of the cell cycle and apoptosis, we have analyzed the levels and subcellular localization of endogenous ß-catenin and its relation with adenomatous polyposis coli (APC) during the cell cycle in S-phase¿synchronized epithelial cells. ß-Catenin levels increase in S phase, reaching maximum accumulation at late G2/M and then abruptly decreasing as the cells enter into a new G1 phase. In parallel, an increased cytoplasmic and nuclear localization of ß-catenin and APC is observed during S and G2 phases. In addition, strong colocalization of APC with centrosomes, but not ß-catenin, is detected in M phase. Interestingly, overexpression of a stable form of ß-catenin, or inhibition of endogenous ß-catenin degradation, in epidermal keratinocyte cells induces a G2 cell cycle arrest and leads to apoptosis. These results support a role for ß-catenin in the control of cell cycle and apoptosis at G2/M in normal and transformed epidermal keratinocytes.
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It is part of: Molecular Biology of the Cell, 2003, vol. 14, núm. 7, p. 2844-2860
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ISSN: 1059-1524
Appears in Collections:Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)

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