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2003-03-11
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/25182

ß-Catenin Regulation during the Cell Cycle: Implications in G2/M and Apoptosis

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http://dx.doi.org/10.1091/mbc.E03-01-0865

Resum

ß-catenin is a multifunctional protein involved in cell-cell adhesion and Wnt signal transduction. ß-Catenin signaling has been proposed to act as inducer of cell proliferation in different tumors. However, in some developmental contexts and cell systems ß-catenin also acts as a positive modulator of apoptosis. To get additional insights into the role of ß-Catenin in the regulation of the cell cycle and apoptosis, we have analyzed the levels and subcellular localization of endogenous ß-catenin and its relation with adenomatous polyposis coli (APC) during the cell cycle in S-phase¿synchronized epithelial cells. ß-Catenin levels increase in S phase, reaching maximum accumulation at late G2/M and then abruptly decreasing as the cells enter into a new G1 phase. In parallel, an increased cytoplasmic and nuclear localization of ß-catenin and APC is observed during S and G2 phases. In addition, strong colocalization of APC with centrosomes, but not ß-catenin, is detected in M phase. Interestingly, overexpression of a stable form of ß-catenin, or inhibition of endogenous ß-catenin degradation, in epidermal keratinocyte cells induces a G2 cell cycle arrest and leads to apoptosis. These results support a role for ß-catenin in the control of cell cycle and apoptosis at G2/M in normal and transformed epidermal keratinocytes.

Matèries

Apoptosi, Cicle cel·lular, Proteïnes citosquelètiques

Matèries (anglès)

Apoptosis, Cell cycle, Cytoskeletal proteins

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Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)

Citació

OLMEDA, David, CASTEL I GIL, Susanna, VILARÓ, Senén, CANO, Amparo. ß-Catenin Regulation during the Cell Cycle: Implications in G2/M and Apoptosis. _Molecular Biology of the Cell_. 2003. Vol. 14, núm. 7, pàgs. 2844-2860. [consulta: 21 de gener de 2026]. ISSN: 1059-1524. [Disponible a: https://hdl.handle.net/2445/25182]

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