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Título: | ß-Catenin Regulation during the Cell Cycle: Implications in G2/M and Apoptosis |
Autor: | Olmeda, David Castel i Gil, Susanna Vilaró, Senén, 1956-2005 Cano, Amparo |
Materia: | Apoptosi Cicle cel·lular Proteïnes citosquelètiques Apoptosis Cell cycle Cytoskeletal proteins |
Fecha de publicación: | 11-mar-2003 |
Publicado por: | American Society for Cell Biology |
Resumen: | ß-catenin is a multifunctional protein involved in cell-cell adhesion and Wnt signal transduction. ß-Catenin signaling has been proposed to act as inducer of cell proliferation in different tumors. However, in some developmental contexts and cell systems ß-catenin also acts as a positive modulator of apoptosis. To get additional insights into the role of ß-Catenin in the regulation of the cell cycle and apoptosis, we have analyzed the levels and subcellular localization of endogenous ß-catenin and its relation with adenomatous polyposis coli (APC) during the cell cycle in S-phase¿synchronized epithelial cells. ß-Catenin levels increase in S phase, reaching maximum accumulation at late G2/M and then abruptly decreasing as the cells enter into a new G1 phase. In parallel, an increased cytoplasmic and nuclear localization of ß-catenin and APC is observed during S and G2 phases. In addition, strong colocalization of APC with centrosomes, but not ß-catenin, is detected in M phase. Interestingly, overexpression of a stable form of ß-catenin, or inhibition of endogenous ß-catenin degradation, in epidermal keratinocyte cells induces a G2 cell cycle arrest and leads to apoptosis. These results support a role for ß-catenin in the control of cell cycle and apoptosis at G2/M in normal and transformed epidermal keratinocytes. |
Nota: | Reproducció del document publicat a: http://dx.doi.org/10.1091/mbc.E03-01-0865 |
Es parte de: | Molecular Biology of the Cell, 2003, vol. 14, núm. 7, p. 2844-2860 |
URI: | https://hdl.handle.net/2445/25182 |
Recurso relacionado: | http://dx.doi.org/10.1091/mbc.E03-01-0865 |
ISSN: | 1059-1524 |
Aparece en las colecciones: | Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia) |
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