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dc.contributor.authorMurphy, Gretchen
dc.contributor.authorMoore, Mary Shannoncat
dc.contributor.authorDrivas, Georgecat
dc.contributor.authorPérez de la Ossa, Pablocat
dc.contributor.authorVillamarin, Aliciacat
dc.contributor.authorD'Eustachio, Peter, 1949-cat
dc.contributor.authorRush, Mark G., 1942-cat
dc.description.abstractRan, the small, predominantly nuclear GTPase, has been implicated in the regulation of a variety of cellular processes including cell cycle progression, nuclear-cytoplasmic trafficking of RNA and protein, nuclear structure, and DNA synthesis. It is not known whether Ran functions directly in each process or whether many of its roles may be secondary to a direct role in only one, for example, nuclear protein import. To identify biochemical links between Ran and its functional target(s), we have generated and examined the properties of a putative Ran effector mutation, T42A-Ran. T42A-Ran binds guanine nucleotides as well as wild-type Ran and responds as well as wild-type Ran to GTP or GDP exchange stimulated by the Ran-specific guanine nucleotide exchange factor, RCC1. T42A-Ran·GDP also retains the ability to bind p10/NTF2, a component of the nuclear import pathway. In contrast to wild-type Ran, T42A-Ran·GTP binds very weakly or not detectably to three proposed Ran effectors, Ran-binding protein 1 (RanBP1), Ran-binding protein 2 (RanBP2, a nucleoporin), and karyopherin ß (a component of the nuclear protein import pathway), and is not stimulated to hydrolyze bound GTP by Ran GTPase-activating protein, RanGAP1. Also in contrast to wild-type Ran, T42A-Ran does not stimulate nuclear protein import in a digitonin permeabilized cell assay and also inhibits wild-type Ran function in this system. However, the T42A mutation does not block the docking of karyophilic substrates at the nuclear pore. These properties of T42A-Ran are consistent with its classification as an effector mutant and define the exposed region of Ran containing the mutation as a probable effector loop.eng
dc.format.extent14 p.-
dc.publisherAmerican Society for Cell Biology-
dc.relation.isformatofReproducció del document publicat a:
dc.relation.ispartofMolecular Biology of the Cell, 1997, vol. 8, núm. 12, p.2591-2604-
dc.rightscc-by-nc-sa, (c) Murphy et al., 1997-
dc.sourceArticles publicats en revistes (Ciències Fisiològiques)-
dc.subject.classificationProteïnes Gcat
dc.subject.classificationTrifosfatasa de guanosinacat
dc.subject.otherG Proteinseng
dc.subject.otherGuanosine triphosphataseeng
dc.titleA T42A Ran Mutation: Differential Interactions with Effectors and Regulators, and Defect in Nuclear Protein Importeng
Appears in Collections:Articles publicats en revistes (Ciències Fisiològiques)

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