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Title: Dynamic and Regulated Association of Caveolin with Lipid Bodies: Modulation of Lipid Body Motility and Function by a Dominant Negative Mutant
Author: Pol i Sorolla, Albert
Martin, Sally
Fernandez, Manuel A.
Ferguson, Charles
Luetterforst, Robert
Enrich Bastús, Carles
Parton, Robert G.
Carozzi, Amanda
Keywords: Proteïnes de membrana
Membrane proteins
Issue Date: 3-Sep-2003
Publisher: American Society for Cell Biology
Abstract: Caveolins are a crucial component of caveolae but have also been localized to the Golgi complex, and, under some experimental conditions, to lipid bodies (LBs). The physiological relevance and dynamics of LB association remain unclear. We now show that endogenous caveolin-1 and caveolin-2 redistribute to LBs in lipid loaded A431 and FRT cells. Association with LBs is regulated and reversible; removal of fatty acids causes caveolin to rapidly leave the lipid body. We also show by subcellular fractionation, light and electron microscopy that during the first hours of liver regeneration, caveolins show a dramatic redistribution from the cell surface to the newly formed LBs. At later stages of the regeneration process (when LBs are still abundant), the levels of caveolins in LBs decrease dramatically. As a model system to study association of caveolins with LBs we have used brefeldin A (BFA). BFA causes rapid redistribution of endogenous caveolins to LBs and this association was reversed upon BFA washout. Finally, we have used a dominant negative LB-associated caveolin mutant (cavDGV) to study LB formation and to examine its effect on LB function. We now show that the cavDGV mutant inhibits microtubule-dependent LB motility and blocks the reversal of lipid accumulation in LBs.
Note: Reproducció del document publicat a:
It is part of: Molecular Biology of the Cell, 2004, vol. 15, núm. 1, p.99-110
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ISSN: 1059-1524
Appears in Collections:Articles publicats en revistes (Biomedicina)

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