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92437.pdf (2.3 MB)

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1995-02-15

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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/25209

The t-SNAREs syntaxin 1 and SNAP-25 are present on organelles that participate in synaptic vesicle recycling

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Recurs relacionat

http://dx.doi.org/10.1083/jcb.128.4.637

Resum

Syntaxin 1 and synaptosome-associated protein of 25 kD (SNAP-25) are neuronal plasmalemma proteins that appear to be essential for exocytosis of synaptic vesicles (SVs). Both proteins form a complex with synaptobrevin, an intrinsic membrane protein of SVs. This binding is thought to be responsible for vesicle docking and apparently precedes membrane fusion. According to the current concept, syntaxin 1 and SNAP-25 are members of larger protein families, collectively designated as target-SNAP receptors (t-SNAREs), whose specific localization to subcellular membranes define where transport vesicles bind and fuse. Here we demonstrate that major pools of syntaxin 1 and SNAP-25 recycle with SVs. Both proteins cofractionate with SVs and clathrin-coated vesicles upon subcellular fractionation. Using recombinant proteins as standards for quantitation, we found that syntaxin 1 and SNAP-25 each comprise approximately 3% of the total protein in highly purified SVs. Thus, both proteins are significant components of SVs although less abundant than synaptobrevin (8.7% of the total protein). Immunoisolation of vesicles using synaptophysin and syntaxin specific antibodies revealed that most SVs contain syntaxin 1. The widespread distribution of both syntaxin 1 and SNAP-25 on SVs was further confirmed by immunogold electron microscopy. Botulinum neurotoxin C1, a toxin that blocks exocytosis by proteolyzing syntaxin 1, preferentially cleaves vesicular syntaxin 1. We conclude that t-SNAREs participate in SV recycling in what may be functionally distinct forms.

Matèries

Neurones, Sinapsi, Neurotransmissió, Interacció cel·lular

Matèries (anglès)

Neurons, Synapses, Neural transmission, Cell interaction

Citació

Col·leccions

Articles publicats en revistes (Patologia i Terapèutica Experimental)

Citació

WALCH-SOLIMENA, Christiane, BLASI CABÚS, Joan, EDELMANN, Lambert, CHAPMANN, Edwin r., FISCHER VON MOLLARD, Gabriele, JAHN, Reinhard. The t-SNAREs syntaxin 1 and SNAP-25 are present on organelles that participate in synaptic vesicle recycling. _Journal of Cell Biology_. 1995. Vol. 128, núm. 4, pàgs. 637-645. [consulta: 25 de desembre de 2025]. ISSN: 0021-9525. [Disponible a: https://hdl.handle.net/2445/25209]

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