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Title: Thyroid hormone regulates reelin and dab1 expression during brain development
Author: Alvarez-Dolado, Manuel
Ruiz Pombo, Mónica
Río Fernández, José Antonio del
Alcántara Horrillo, Soledad
Burgaya i Márquez, Ferran
Sheldon, Michael
Nakajima, Kazunori
Bernal, Juan
Howell, Brian W.
Curran, Tom
Soriano García, Eduardo
Muñoz, Alberto
Keywords: Teixit nerviós
Hormones tiroides
Nerve tissue
Thyroid hormones
Issue Date: 1999
Publisher: Society for Neuroscience
Abstract: The reelin and dab1 genes are necessary for appropriate neuronal migration and lamination during brain development. Since these processes are controlled by thyroid hormone, we studied the effect of thyroid hormone deprivation and administration on the expression of reelin anddab1. As shown by Northern analysis, in situ hybridization, and immunohistochemistry studies, hypothyroid rats expressed decreased levels of reelinRNA and protein during the perinatal period [embryonic day 18 (E18) and postnatal day 0 (P0)]. The effect was evident in Cajal-Retzius cells of cortex layer I, as well as in layers V/VI, hippocampus, and granular neurons of the cerebellum. At later ages, however, Reelin was more abundant in the cortex, hippocampus, cerebellum, and olfactory bulb of hypothyroid rats (P5), and no differences were detected at P15. Conversely, Dab1 levels were higher at P0, and lower at P5 in hypothyroid animals. In line with these results, reelin RNA and protein levels were higher in cultured hippocampal slices from P0 control rats compared to those from hypothyroid animals. Significantly, thyroid-dependent regulation of reelin anddab1 was confirmed in vivo and in vitro by hormone treatment of hypothyroid rats and organotypic cultures, respectively. In both cases, thyroid hormone led to an increase in reelin expression. Our data suggest that the effects of thyroid hormone on neuronal migration may be in part mediated through the control of reelin anddab1 expression during brain ontogenesis.
Note: Reproducció del document publicat a:
It is part of: The Journal of Neuroscience, 1999, vol. 19, num. 16, p. 6979-6993
ISSN: 0270-6474
Appears in Collections:Articles publicats en revistes (Patologia i Terapèutica Experimental)
Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)
Articles publicats en revistes (Medicina)

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