Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/32319
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dc.contributor.authorMartínez Ocaña, Verónica-
dc.contributor.authorUgartondo Casadevall, Vanessa-
dc.contributor.authorVinardell Martínez-Hidalgo, Ma. Pilar-
dc.contributor.authorTorres Simón, Josep Lluís-
dc.contributor.authorMitjans Arnal, Montserrat-
dc.date.accessioned2012-10-15T14:09:55Z-
dc.date.available2013-12-31T23:01:53Z-
dc.date.issued2012-04-05-
dc.identifier.issn0021-8561-
dc.identifier.urihttp://hdl.handle.net/2445/32319-
dc.description.abstractEpicatechin conjugates obtained from grape have shown antioxidant activity in various systems. However, how these conjugates exert their antioxidant benefits has not been widely studied. We assessed the activity of epicatechin and epicatechin conjugates on the erythrocyte membrane in the presence and absence of a peroxyl radical initiator, to increase our understanding of their mechanisms. Thus, we studied cell membrane fluidity by fluorescence anisotropy measurements, morphology of erythrocytes by scanning electron microscopy, and finally, red cell membrane proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Our data showed that incubation of red cells in the presence of epicatechin derivatives altered membrane fluidity and erythrocyte morphology but not the membrane protein pattern. The presence in the medium of the peroxyl radical initiator 2,2′-azobis(amidinopropane) dihydrochloride (AAPH) resulted in membrane disruptions at all levels analyzed, causing changes in membrane fluidity, cell morphology, and protein degradation. The presence of antioxidants avoided protein oxidation, indicating that the interaction of epicatechin conjugates with the lipid bilayer might reduce the accessibility of AAPH to membranes, which could explain in part the inhibitory ability of these compounds against hemolysis induced by peroxidative insult.-
dc.format.extent6 p.-
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherAmerican Chemical Society, Books and Journals Division-
dc.relation.isformatofVersió postprint del document publicat a: http://dx.doi.org/10.1021/jf2051784-
dc.relation.ispartofJournal of Agricultural and Food Chemistry, 2012, vol. 60, num. 16, p. 4090-4095-
dc.relation.urihttp://dx.doi.org/10.1021/jf2051784-
dc.rights(c) American Chemical Society, Books and Journals Division], 2012-
dc.sourceArticles publicats en revistes (Bioquímica i Fisiologia)-
dc.subject.classificationPolifenols-
dc.subject.classificationAntioxidants-
dc.subject.classificationRaïms-
dc.subject.classificationHematies-
dc.subject.classificationProteïnes de membrana-
dc.subject.otherPolyphenols-
dc.subject.otherAntioxidants-
dc.subject.otherGrapes-
dc.subject.otherErythrocytes-
dc.subject.otherMembrane proteins-
dc.titleGrape epicatechin conjugates prevent erythrocyte membrane protein oxidation-
dc.typeinfo:eu-repo/semantics/article-
dc.typeinfo:eu-repo/semantics/acceptedVersion-
dc.identifier.idgrec603043-
dc.date.updated2012-10-04T17:42:36Z-
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess-
dc.identifier.pmid22480260-
Appears in Collections:Articles publicats en revistes (Bioquímica i Fisiologia)

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