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Title: Assessment of the potential skin irritation of lysine-derivative anionic surfactants using mouse fibroblast and human keratinocytes as an alternative to animal testing
Author: Sánchez Molina, Lourdes
Mitjans Arnal, Montserrat
Infante Martínez-Pardo, Ma. Rosa
Vinardell Martínez-Hidalgo, Ma. Pilar
Keywords: Agents tensioactius
Cultiu cel·lular
Estructura molecular
Surface active agents
Amino acids
Cell culture
Molecular structure
Issue Date: Sep-2004
Publisher: Springer Science + Business Media
Abstract: Purpose. The aim of this study was to identify new surfactants with low skin irritant properties for use in pharmaceutical and cosmetic formulations, employing cell culture as an alternative method to in vivo testing. In addition, we sought to establish whether potential cytotoxic properties were related to the size of the counterions bound to the surfactants. Methods. Cytotoxicity was assessed in the mouse fibroblast cell line 3T6, and the human keratinocyte cell line NCTC 2544, using the MTT assay and uptake of the vital dye neutral red 24 h after dosing (NRU). Results. Lysine-derivative surfactants showed higher IC50s than did commercial anionic irritant compounds such as sodium dodecyl sulphate, proving to be no more harmful than amphoteric betaines. The aggressiveness of the surfactants depended upon the size of their constituent counterions: surfactants associated with lighter counterions showed a proportionally higher aggressivity than those with heavier ones. Conclusions. Synthetic lysine-derivative anionic surfactants are less irritant than commercial surfactants such as sodium dodecyl sulphate and Hexadecyltrimethylammonium bromide and are similar to Betaines. These surfactants may offer promising applications in pharmaceutical and cosmetic preparations, representing a potential alternative to commercial anionic surfactants as a result of their low irritancy potential.
Note: Versió postprint del document publicat a:
It is part of: Pharmaceutical Research, 2004, vol. 21, num. 9, p. 1637-1641
Related resource:
ISSN: 0724-8741
Appears in Collections:Articles publicats en revistes (Bioquímica i Fisiologia)

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