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Title: Comparative sensitivity of tumor and non-tumor cell lines as reliable approach for in vitro cytotoxicity screening of lysine-based surfactants with potential pharmaceutical applications
Author: Nogueira, Daniele R.
Mitjans Arnal, Montserrat
Infante Martínez-Pardo, Ma. Rosa
Vinardell Martínez-Hidalgo, Ma. Pilar
Keywords: Agents tensioactius
Cèl·lules canceroses
Tests de toxicitat
Citotoxicitat per mediació cel·lular
Surface active agents
Cancer cells
Toxicity testing
Cell-mediated cytotoxicity
Biological assay
Issue Date: 25-Nov-2011
Publisher: Elsevier B.V.
Abstract: Surfactants are used as additives in topical pharmaceuticals and drug delivery systems. The biocompatibility of amino acid-based surfactants makes them highly suitable for use in these fields, but tests are needed to evaluate their potential toxicity. Here we addressed the sensitivity of tumor (HeLa, MCF-7) and non-tumor (3T3, 3T6, HaCaT, NCTC 2544) cell lines to the toxic effects of lysine-based surfactants by means of two in vitro endpoints (MTT and NRU). This comparative assay may serve as a reliable approach for predictive toxicity screening of chemicals prior to pharmaceutical applications. After 24-h of cell exposure to surfactants, differing toxic responses were observed. NCTC 2544 and 3T6 cell lines were the most sensitive, while both tumor cells and 3T3 fibroblasts were more resistant to the cytotoxic effects of surfactants. IC50-values revealed that cytotoxicity was detected earlier by MTT assay than by NRU assay, regardless of the compound or cell line. The overall results showed that surfactants with organic counterions were less cytotoxic than those with inorganic counterions. Our findings highlight the relevance of the correct choice and combination of cell lines and bioassays in toxicity studies for a safe and reliable screen of chemicals with potential interest in pharmaceutical industry.
Note: Versió postprint del document publicat a:
It is part of: International Journal of Pharmaceutics, 2011, vol. 420, núm. 1, p. 51-58
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ISSN: 0378-5173
Appears in Collections:Articles publicats en revistes (Bioquímica i Fisiologia)

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