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Title: The membrane-permeable calmodulin inhibitors (W-7/W-13) bind to membranes changing the electrostatic surface potential. Dual effect of W-13 on EGFR activation
Author: Sengupta, Parijat
Ruano, María José
Tebar Ramon, Francesc
Golebiewska, Urszula
Zaitseva, Irina
Enrich Bastús, Carles
McLaughlin, Stuart
Villalobo, Antonio
Keywords: Calmodulina
Factor de creixement epidèrmic
Epidermal growth factor
Issue Date: 16-Mar-2007
Publisher: American Society for Biochemistry and Molecular Biology
Abstract: Membrane-permeable calmodulin inhibitors, such as the napthalenesulfonamide derivatives W-7/W-13, trifluoperazine, and calmidazolium, are used widely to investigate the role of calcium/calmodulin (Ca2+/CaM) in living cells. If two chemically different inhibitors (e.g. W-7 and trifluoperazine) produce similar effects, investigators often assume the effects are due to CaM inhibition. Zeta potential measurements, however, show that these amphipathic weak bases bind to phospholipid vesicles at the same concentrations as they inhibit Ca 2 /CaM; this suggests that they also bind to the inner leaflet of the plasma membrane, reducing its negative electrostatic surface potential. This change will cause electrostatically bound clusters of basic residues on peripheral (e.g. Src and K-Ras4B) and integral (e.g. epidermal growth factor receptor (EGFR)) proteins to translocate from the membrane to the cytoplasm. We measured inhibitor-mediated translocation of a simple basic peptide corresponding to the calmodulin-binding juxtamembrane region of the EGFR on model membranes; W-7/W-13 causes translocation of this peptide from membrane to solution, suggesting that caution must be exercised when interpreting the results obtained with these inhibitors in living cells. We present evidence that they exert dual effects on autophosphorylation of EGFR;W-13 inhibits epidermal growth factordependent EGFR autophosphorylation under different experimental conditions, but in the absence of epidermal growth factor, W-13 stimulates autophosphorylation of the receptor in four different cell types. Our interpretation is that the former effect is due toW-13inhibition of Ca 2 /CaM, but thelatter results could be due to binding of W-13 to the plasma membrane.
Note: Versió postprint del document publicat a: 10.1074/jbc.M607211200
It is part of: Journal of Biological Chemistry, 2007, vol. 282, num. 11, p. 8474-8486
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ISSN: 0021-9258
Appears in Collections:Articles publicats en revistes (Biomedicina)

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