Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorCliment, Núria-
dc.contributor.authorGuerra, Susana-
dc.contributor.authorGarcía Alcaide, Felipe-
dc.contributor.authorRovira Ollé, Cristina-
dc.contributor.authorMiralles Escofet, Laia-
dc.contributor.authorGómez Rodríguez, Carmen Elena-
dc.contributor.authorPiqué i Clusella, Núria-
dc.contributor.authorGil Roda, Cristina-
dc.contributor.authorGatell, José M.-
dc.contributor.authorEsteban Rodrígez, Mariano-
dc.contributor.authorGallart, Teresa-
dc.description.abstractCurrently, MVA virus vectors carrying HIV-1 genes are being developed as HIV-1/AIDS prophylactic/therapeutic vaccines. Nevertheless, little is known about the impact of these vectors on human dendritic cells (DC) and their capacity to present HIV-1 antigens to human HIV-specific T cells. This study aimed to characterize the interaction of MVA and MVA expressing the HIV-1 genes Env-Gag-Pol-Nef of clade B (referred to as MVA-B) in human monocyte-derived dendritic cells (MDDC) and the subsequent processes of HIV-1 antigen presentation and activation of memory HIV-1-specific T lymphocytes. For these purposes, we performed ex vivo assays with MDDC and autologous lymphocytes from asymptomatic HIV-infected patients. Infection of MDDC with MVA-B or MVA, at the optimal dose of 0.3 PFU/MDDC, induced by itself a moderate degree of maturation of MDDC, involving secretion of cytokines and chemokines (IL1-ra, IL-7, TNF-α, IL-6, IL-12, IL-15, IL-8, MCP-1, MIP-1α, MIP-1β, RANTES, IP-10, MIG, and IFN-α). MDDC infected with MVA or MVA-B and following a period of 48 h or 72 h of maturation were able to migrate toward CCL19 or CCL21 chemokine gradients. MVA-B infection induced apoptosis of the infected cells and the resulting apoptotic bodies were engulfed by the uninfected MDDC, which cross-presented HIV-1 antigens to autologous CD8+ T lymphocytes. MVA-B-infected MDDC co-cultured with autologous T lymphocytes induced a highly functional HIV-specific CD8+ T cell response including proliferation, secretion of IFN-γ, IL-2, TNF-α, MIP-1β, MIP-1α, RANTES and IL-6, and strong cytotoxic activity against autologous HIV-1-infected CD4+ T lymphocytes. These results evidence the adjuvant role of the vector itself (MVA) and support the clinical development of prophylactic and therapeutic anti-HIV vaccines based on MVA-B.-
dc.format.extent17 p.-
dc.publisherPublic Library of Science (PLoS)-
dc.relation.isformatofReproducció del document publicat a:
dc.relation.ispartofPLoS One, 2011, vol. 6, num. 5, p. e19644-
dc.rightscc-by (c) Climent i Vidal, Núria et al., 2011-
dc.subject.classificationVacunes antivíriques-
dc.subject.classificationInfeccions per VIH-
dc.subject.classificationCèl·lules T-
dc.subject.classificationVirus ADN-
dc.subject.otherViral vaccines-
dc.subject.otherHIV infections-
dc.subject.otherT cells-
dc.subject.otherDNA viruses-
dc.titleDendritic cells exposed to MVA-based HIV-1 vaccine induce highly functional HIV-1-specific CD8+ T cell responses in HIV-1-infected individuals-
Appears in Collections:Articles publicats en revistes (Medicina)
Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)

Files in This Item:
File Description SizeFormat 
595273.pdf1.93 MBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons