Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/43057
Title: | Uridine metabolism in HIV-1-infected patients: effect of infection, of antiretroviral therapy and of HIV-1/ART-associated lypodystrophy syndrome. |
Author: | Domingo, Pere (Domingo Pedrol) Torres Torronteras, Javier Pomar Solchaga, Virginia Giralt i Oms, Marta Domingo i Pedrol, Joan Carles Gutiérrez, Maria del Mar Gallego Escuredo, José Miguel Mateo, Maria Gracia Cano Soldado, Pedro Javier Fernández, Irene Pastor Anglada, Marçal Vidal, Francesc Villarroya i Gombau, Francesc Andreu, Antoni Martí, Ramon |
Keywords: | VIH (Virus) Antiretrovirals Síndrome de lipodistròfia associada a VIH Metabolisme HIV (Viruses) Antiretroviral agents HIV-associated lipodystrophy syndrome Metabolism |
Issue Date: | 2010 |
Publisher: | Public Library of Science (PLoS) |
Abstract: | Background Uridine has been advocated for the treatment of HIV-1/HAART-associated lipodystrophy (HALS), although its metabolism in HIV-1-infected patients is poorly understood. Methods Plasma uridine concentrations were measured in 35 controls and 221 HIV-1-infected patients and fat uridine in 15 controls and 19 patients. The diagnosis of HALS was performed following the criteria of the Lipodystrophy Severity Grading Scale. Uridine was measured by a binary gradient-elution HPLC method. Analysis of genes encoding uridine metabolizing enzymes in fat was performed with TaqMan RT-PCR. Results Median plasma uridine concentrations for HIV-1-infected patients were 3.80 µmol/l (interquartile range: 1.60), and for controls 4.60 µmol/l (IQR: 1.8) (P = 0.0009). In fat, they were of 6.0 (3.67), and 2.8 (4.65) nmol/mg of protein, respectively (P = 0.0118). Patients with a mixed HALS form had a median plasma uridine level of 4.0 (IC95%: 3.40-4.80) whereas in those with isolated lipoatrophy it was 3.25 (2.55-4.15) µmol/l/l (P = 0.0066). The expression of uridine cytidine kinase and uridine phosphorylase genes was significantly decreased in all groups of patients with respect to controls. A higher expression of the mRNAs for concentrative nucleoside transporters was found in HIV-1-infected patients with respect to healthy controls. Conclusions HIV-1 infection is associated with a decrease in plasma uridine and a shift of uridine to the adipose tissue compartment. Antiretroviral therapy was not associated with plasma uridine concentrations, but pure lipoatrophic HALS was associated with significantly lower plasma uridine concentrations. |
Note: | Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0013896 |
It is part of: | PLoS One, 2010, vol. 5, num. 11, p. e13896 |
URI: | http://hdl.handle.net/2445/43057 |
Related resource: | http://dx.doi.org/10.1371/journal.pone.0013896 |
ISSN: | 1932-6203 |
Appears in Collections: | Articles publicats en revistes (Bioquímica i Biomedicina Molecular) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
609733.pdf | 332.31 kB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License