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Title: Protein interaction studies point to new functions for Escherichia coli glyceraldehyde-3-phosphate dehydrogenase
Author: Ferreira, Elaine
Giménez Claudio, Rosa
Aguilera Gil, Maria Laura
Guzmán López, Karla
Aguilar Piera, Juan
Badía Palacín, Josefa
Baldomà Llavinés, Laura
Keywords: Proteïnes
Escheríchia coli
Reparació de l'ADN
Escherichia coli
DNA repair
Issue Date: 1-Feb-2013
Publisher: Elsevier Masson SAS
Abstract: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is considered a multifunctional protein with defined functions in numerous mammalian cellular processes. GAPDH functional diversity depends on various factors such as covalent modifications, subcellular localization, oligomeric state and intracellular concentration of substrates or ligands, as well as protein-protein interactions. In bacteria, alternative GAPDH functions have been associated with its extracellular location in pathogens or probiotics. In this study, new intracellular functions of E. coli GAPDH were investigated following a proteomic approach aimed at identifying interacting partners using in vivo formaldehyde cross-linking followed by mass spectrometry. The identified proteins were involved in metabolic processes, protein synthesis and folding or DNA repair. Some interacting proteins were also identified in immunopurification experiments in the absence of cross-linking. Pull-down experiments and overlay immunoblotting were performed to further characterize the interaction with phosphoglycolate phosphatase (Gph). This enzyme is involved in the metabolism of 2-phosphoglycolate formed in the DNA repair of 3"-phosphoglycolate ends generated by bleomycin damage. We show that interaction between Gph and GAPDH increases in cells challenged with bleomycin, suggesting involvement of GAPDH in cellular processes linked to DNA repair mechanisms.
Note: Versió postprint del document publicat a:
It is part of: Research in Microbiology, 2013, vol. 164, p. 145-154
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ISSN: 0923-2508
Appears in Collections:Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

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