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http://hdl.handle.net/2445/43964
Title: | Ras-association domain of sorting nexin 27 is critical for regulating expression of GIRK potassium channels |
Author: | Bahima Borràs, Laia Balana, Bartosz Bodhinathan, Karthik Taura, Jaume Taylor, Natalie M. Nettleton, Margaret Y. Ciruela Alférez, Francisco Slesinger, Paul A. |
Keywords: | Proteïnes ras Canals de potassi Proteïnes G Ras proteins Potassium channels G Proteins |
Issue Date: | 26-Feb-2013 |
Publisher: | Public Library of Science (PLoS) |
Abstract: | G protein-gated inwardly rectifying potassium (GIRK) channels play an important role in regulating neuronal excitability. Sorting nexin 27b (SNX27b), which reduces surface expression of GIRK channels through a PDZ domain interaction, contains a putative Ras-association (RA) domain with unknown function. Deleting the RA domain in SNX27b (SNX27b-DRA) prevents the down-regulation of GIRK2c/GIRK3 channels. Similarly, a point mutation (K305A) in the RA domain disrupts regulation of GIRK2c/GIRK3 channels and reduces H-Ras binding in vitro. Finally, the dominant-negative H-Ras (S17N) occludes the SNX27b-dependent decrease in surface expression of GIRK2c/GIRK3 channels. Thus, the presence of a functional RA domain and the interaction with Ras-like G proteins comprise a novel mechanism for modulating SNX27b control of GIRK channel surface expression and cellular excitability. |
Note: | Reproducció del document publicat a: http://doi.org/10.1371/journal.pone.0059800 |
It is part of: | PLoS One, 2013, vol. 8, num. 3, p. e59800 |
URI: | http://hdl.handle.net/2445/43964 |
Related resource: | http://doi.org/10.1371/journal.pone.0059800 |
ISSN: | 1932-6203 |
Appears in Collections: | Articles publicats en revistes (Patologia i Terapèutica Experimental) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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