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http://hdl.handle.net/2445/44124
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DC Field | Value | Language |
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dc.contributor.author | Barragán, Flavia | - |
dc.contributor.author | López-Senín, Paula | - |
dc.contributor.author | Salassa, Luca | - |
dc.contributor.author | Betanzos-Lara, Soledad | - |
dc.contributor.author | Habtemariam, Abraha | - |
dc.contributor.author | Moreno Martínez, Virtudes | - |
dc.contributor.author | Sadler, Peter | - |
dc.contributor.author | Marchán Sancho, Vicente | - |
dc.date.accessioned | 2013-06-07T11:21:35Z | - |
dc.date.available | 2013-06-07T11:21:35Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0002-7863 | - |
dc.identifier.uri | http://hdl.handle.net/2445/44124 | - |
dc.description.abstract | A photoactivated ruthenium(II) arene complex has been conjugated to two receptor-binding peptides, a dicarba analogue of octreotide and the Arg-Gly-Asp (RGD) tripeptide. These peptides can act as"tumor-targeting devices" since their receptors are overexpressed on the membranes of tumor cells. Both ruthenium-peptide conjugates are stable in aqueous solution in the dark, but upon irradiation with visible light, the pyridyl-derivatized peptides were selectively photodissociated from the ruthenium complex, as inferred by UV-vis and NMR spectroscopy. Importantly, the reactive aqua species generated from the conjugates, [(η6-p-cym)Ru(bpm)(H2O)]2+, reacted with the model DNA nucleobase 9-ethylguanine as well as with guanines of two DNA sequences, 5′dCATGGCT and 5′dAGCCATG. Interestingly, when irradiation was performed in the presence of the oligonucleotides, a new ruthenium adduct involving both guanines was formed as a consequence of the photodriven loss of p-cymene from the two monofunctional adducts. The release of the arene ligand and the formation of a ruthenated product with a multidentate binding mode might have important implications for the biological activity of such photoactivated ruthenium(II) arene complexes. Finally, photoreactions with the peptide-oligonucleotide hybrid, Phac-His-Gly-Met-linker-p5′dCATGGCT, also led to arene release and to guanine adducts, including a GG chelate. The lack of interaction with the peptide fragment confirms the preference of such organometallic ruthenium(II) complexes for guanine over other potential biological ligands, such as histidine or methionine amino acids. | - |
dc.format.extent | 11 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | American Chemical Society | - |
dc.relation.isformatof | Versió postprint del document publicat a: http://dx.doi.org/10.1021/ja205235m | - |
dc.relation.ispartof | Journal of the American Chemical Society, 2011, vol. 133, num. 35, p. 14098-14108 | - |
dc.relation.uri | http://dx.doi.org/10.1021/ja205235m | - |
dc.rights | (c) American Chemical Society , 2011 | - |
dc.source | Articles publicats en revistes (Química Inorgànica i Orgànica) | - |
dc.subject.classification | Quimioteràpia del càncer | - |
dc.subject.classification | Pèptids | - |
dc.subject.classification | Ruteni | - |
dc.subject.classification | Oligonucleòtids | - |
dc.subject.classification | Receptors cel·lulars | - |
dc.subject.other | Cancer chemotherapy | - |
dc.subject.other | Peptides | - |
dc.subject.other | Ruthenium | - |
dc.subject.other | Oligonucleotides | - |
dc.subject.other | Cell receptors | - |
dc.title | Photocontrolled DNA Binding of a Receptor-Targeted Organometallic Ruthenium(II) Complex | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 599372 | - |
dc.date.updated | 2013-06-07T11:21:35Z | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/220281/EU//PHOTORUACD | - |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/247450/EU//BIOINCMED | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Química Inorgànica i Orgànica) Publicacions de projectes de recerca finançats per la UE |
Files in This Item:
File | Description | Size | Format | |
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599372.pdf | 1.69 MB | Adobe PDF | View/Open |
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